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Alteration of global transcription by saccharin in Acinetobacter baumannii AB5075

ABSTRACT: Alteration of global transcription by saccharin in Acinetobacter baumannii AB5075

PROVIDER: PRJNA998336 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25411558_1.fastq.gz	Fastqsanger.gz
	SRR25411558_2.fastq.gz	Fastqsanger.gz
	SRR25411559_1.fastq.gz	Fastqsanger.gz
	SRR25411559_2.fastq.gz	Fastqsanger.gz
	SRR25411560_1.fastq.gz	Fastqsanger.gz

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Alteration of global transcription by saccharin in Acinetobacter baumannii AB5075

Project description:We perform RNA-seq comparing a treatment with 1% saccharin to a mock treatment in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this artificial sweetener. A. baumannii AB5075 cultures were grown in LB medium in the presence of 1% saccharin or a water control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 165 genes appeared upregulated in the presence of saccharin, whereas 215 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to pili and frimbriae biogenesis, involved in biofilms formation, adhesion and twitching motility.

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Alteration of global transcription by the phytochemical Kaempferol in Acinetobacter baumannii AB5075

Project description:We perform RNA-seq comparing a treatment with 0.375 mM Kaempferol to a mock treatment (DMSO) in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this phytochemical. A. baumannii AB5075 cultures were grown in LB medium in the presence of 0.375 mM Kaempferol or a DMSO control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlater for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 99 genes appeared upregulated in the presence of Kaempferol whereas 18 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to iron acquisition, siderophore biosynthesis and iron transport genes.

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native Tn-seq analysis of ISAba13 in Acinetobacter baumannii AB5075

Project description:The experiment contains native Tn-seq data for Acinetobacter baumannii strain AB5075 with different genetic alterations. The strain was grown at 37 degrees in LB medium and genomic DNA was isolated. We then used PCR to select for DNA regions containing a junction between ISAba13 and chromosomal DNA. Libraries were then prepared using these DNA fragments.

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Alteration of global transcription by the phytochemical Kaempferol in Acinetobacter baumannii AB5075

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Alteration of global transcription by the artificial sweetener acesulfame K in Acinetobacter baumannii AB5075

Project description:We perform RNA-seq comparing a treatment with 1.33% acesulfame K to a mock treatment in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this artificial sweetener. A. baumannii AB5075 cultures were grown in LB medium in the presence of 1.33% acesulfame K or a water control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 212 genes appeared upregulated in the presence of acesulfame K, whereas 252 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to twtching motility and natural transformation (downregulation of genes involves in biogenesis and control of Type IV pili and com genes), as well as genes involved in iron acquisition and siderophore biosynthesis and an uncharacterised gene cluster that might be involved in detoxification of sulfonamide compounds.

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