Project description:Genome wide DNA methylation profiling of glioma patient surgical samples and corresponding xenotransplants and cell lines. The Illumina Infinium 450k Human DNA methylation Beadchip and the Illumina EPIC Beadchip were used to obtain DNA methylation profiles. Samples include 22 patient surgical samples, 26 xenografted patient tumors, and 6 cell lines derived there-of.
Project description:Primary outcome(s): The incidence of surgical site infections after laparoscopic colorectal surgery using monofilament absorbable suture material within 30 days after surgery
Project description:Interventions: oral antibiotics
Primary outcome(s): The rate of surgical site infection at 30th days after surgery
Study Design: Single arm Non-randomized
Project description:4 Adult male Sprague-Dawley rats (275-350 g) were anesthetized and subjected to hepatectomy sham surgery (abdominal cavity was opened, liver was handled, but no tissue resection was made). 1 hour after the surgery rats were killed and liver samples were harvested. This study was conducted to analyzes the effects of surgical stress on gene expression levels in rat liver. It provides additional data to 1-6 h partial hepatectomy study (Series GSE7415). Keywords: 1h hepatectomy sham surgery
Project description:Surgical revision of infection can involve multiple procedures. Each revision activates a tissue injury response and disrupts the established bacterial biofilm. However, it is not well understood how the bacteria, immune system, and overall tissue environment coordinate their response to revision. Our results show that immunological niches already compromised by infection – such as the bone marrow, lymph nodes, and circulating blood – further upregulate pro-inflammatory programs in response to revision surgery. This enhanced inflammation at the incision site has no effect on reducing bacteria numbers. Instead, it leads to increased expression of virulence factors, enhanced tissue remodeling, and damage including bone osteolysis and muscle fibrosis. Whereas muscle fibrosis appears to resolve 14 days post-revision, osteolysis continues to progress. These findings show that revision surgery negatively impacts all tissues within the surgical site. It is therefore essential to question the risks and benefits of each revision on a case-by-case basis. Further, understanding the timing and tissue changes associated with revision of infected orthopedic sites will help inform the design of additional interventions to minimize tissue damage and maximize bactericidal effects.
Project description:Interventions: oral antibiotics
Primary outcome(s): The rate of surgical site infection at 30th days after surgery
Study Design: Single arm Non-randomized