Sort   by:  
 Page size 
ProteomicsProtein precipitation of diluted samples with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach
Proteomic approaches are extremely valuable in many fields of research, where mass spectrometry methods have gained an increasing interest especially due to the ability to perform quantitative analysis. Nonetheless, sample preparation prior to mass spectrometry analysis is of the utmost importance. ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-25 | PXD003653 | Pride
Protein precipitation  acetone  tca/acetone  swath 
TranscriptomicsAcetone stress response in Desulfovibrio vulgaris
Desulfovibrio vulgaris has been studied extensively for its potential in the bioremediation of heavy metals and radionuclides. Hydrocarbons and solvents, as frequent environmental co-contaminants, have been reported to inhibit microbial activities and thereby pose a limitation on bioremediation effi...
ORGANISM(S): Desulfovibrio vulgaris str. Hildenborough 
2009-08-08 | E-GEOD-5569 | biostudies-arrayexpress
TranscriptomicsIntegrative analysis of the biodegradation of acetone/isopropanol by Cupriavidus metallidurans CH34: proposal of a metabolic pathway
Various environmental bacteria were adapted to the presence of toxic and recalcitrant organic solvents. Cupriavidus metallidurans CH34, a β-proteobacterium that was found in industrial environments is known as a bacterial model to study heavy metal resistance. Interestingly, genome screening of CH3...
ORGANISM(S): Cupriavidus metallidurans CH34 
2015-08-31 | E-GEOD-33578 | biostudies-arrayexpress
MetabolomicsSearching for metabolic changes in urine headspace composition as an effect of strenuous walking
The present investigation uses proton transfer reaction mass spectrometry (PTR-MS) combined with multivariate and univariate statistical analyses to study potential biomarkers for altered metabolism in urine due to strenuous walking. Urine samples, in concurrence with breath and blood samples, were ...
2016-12-14 | MTBLS164 | MetaboLights
TranscriptomicsAcetone stress response in Desulfovibrio vulgaris
Desulfovibrio vulgaris has been studied extensively for its potential in the bioremediation of heavy metals and radionuclides. Hydrocarbons and solvents, as frequent environmental co-contaminants, have been reported to inhibit microbial activities and thereby pose a limitation on bioremediation effi...
ORGANISM(S): Nitratidesulfovibrio vulgaris str. Hildenborough 
2009-08-08 | GSE5569 | GEO
MetabolomicsNMR-based metabolomic profile of hypercholesterolemic human sera: Relationship with in vitro gene expression?
Hypercholesterolaemia is considered an important cause of atherosclerotic cardiovascular disease. In a previous investigation, we demonstrated that cultured hepatoma cells treated with hypercholesterolaemic sera compared with cells treated with normocholesterolaemic sera show overexpression of mRNAs...
2020-05-26 | MTBLS1416 | MetaboLights
TranscriptomicsIntegrative analysis of the biodegradation of acetone/isopropanol by Cupriavidus metallidurans CH34: proposal of a metabolic pathway
Various environmental bacteria were adapted to the presence of toxic and recalcitrant organic solvents. Cupriavidus metallidurans CH34, a β-proteobacterium that was found in industrial environments is known as a bacterial model to study heavy metal resistance. Interestingly, genome screening of CH34...
ORGANISM(S): Cupriavidus metallidurans CH34 
2015-08-31 | GSE33578 | GEO
TranscriptomicsTranscriptome and microRNA array analyses reveal a stimulatory effect of the phytochemical shikonin on epithelial–mesenchymal transition (EMT) in mouse skin [microRNA expression profile]
We investigate and define here the in vivo biological effects of shikonin, at the transcriptome and microRNA levels, in mouse skin tissues. Through cross-examination between total RNA transcripts and microRNA data sets, we predicted that shikonin treatment may affect the epithelial–mesenchymal trans...
ORGANISM(S): Mus musculus 
2011-12-31 | E-GEOD-32695 | biostudies-arrayexpress
TranscriptomicsFenclorim safening of rice
The herbicide safener fenclorim is used to protect rice from damage by the herbicide pretilachlor, whilst the structural analogue 4-chloro-6-methyl-2-phenylpyrimidine (CMP) is unable safen rice. Fenclorim and CMP were applied to rice cultures to determine differences in the transcriptional response...
ORGANISM(S): Oryza sativa Japonica Group 
2017-07-18 | E-MTAB-5524 | biostudies-arrayexpress
GenomicsSmall RNA next generation sequencing of mice skin biopsies from acetone-treated healthy skins and DMBA/PMA-treated hyperplastic skins, papillomas and cutaneous Squamous Cell Carcinomas
FVB/N mice were subjected to 7,12-Dimethylbenz[a]anthracene (DMBA) two-hit multistage skin carcinogenesis protocol. Mice were topically treated with 200 nmol of DMBA in 0.2 mL acetone then twice weekly for six weeks with 5 nmol PMA (Phorbol 12-myristate 13-acetate). A second hit of DMBA was performe...
ORGANISM(S): Mus musculus 
2014-02-03 | GSE52299 | GEO
Sort   by:  
 Page size