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ProteomicsOnline-2D nanoLC-MS for crude serum proteome profiling: assessing sample preparation impact on proteome composition
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-09 | PXD024893 | Pride
Peptide fractionation Blood Nanolc-ms 1d nanolc-ms Proteome profiling Crude serum High-resolution accurate mass-spectrometry Online-2d nanolc-ms 
ProteomicsMapping differences in salivary gland secretions using dried saliva spots
Dried saliva spot (DSS) sampling is a minimally invasive extraction technique that allows for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nanoLC-Orbitrap-MS/MS analysis, we used DSS to compare the unstimulated proteomes secreted by the paro...
ORGANISM(S): Homo sapiens (Human) 
2019-09-04 | PXD012792 | Pride
Saliva Gland secretion Human Proteome 
ProteomicsIdentification of celiac-disease active peptides in gluten-free beers by nanoLC-MS/MS
Barley beers may be rendered gluten-free by specialized brewing processes. One controversial issue is whether these beers contain residual gluten peptides carrying at least one celiac disease (CeD)-active epitope, because routine methods to assess whether the beer is gluten-free like competitive enz...
ORGANISM(S): Triticum aestivum (Wheat) Hordeum vulgare (Barley) 
2026-04-01 | PXD059719 | Pride
UnknownLarge Scale Screening and Quantitative Analysis of Site-Specific N-Glycopeptides from Human Serum in Early Alzheimer's Disease Using LC-HCD-PRM-MS.
Not available
S-EPMC10289803 | biostudies-literature
UnknownA High-Sensitivity Low-Nanoflow LC-MS Configuration for High-Throughput Sample-Limited Proteomics.
Not available
S-EPMC10753523 | biostudies-literature
ProteomicsLabel-free comparative data independent (DIA) nanoLC-MS/MS analysis of protein expression profiles of mouse primary dendritic cells (DC) from B6 mice on normal and high fructose/high fat (HFHF) diet
A diet rich in saturated fat and carbohydrates causes a low-grade chronic inflammation in several organs including nonalcoholic steatohepatitis. The environment-driven lipotocicity and glucotoxicity induce tissue damage, which promotes dendritic cell adjuvanticity, and supports an MHC-class-II immun...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-22 | PXD026190 | Pride
Mouse dendritic cells (cd11c+) proteomics  dia/dda nano lc-ms/ms orbitrap fusion tribrid mass spectrometer 
ProteomicsNanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL
Chroococcidiopsis thermalis is an extremophilic cyanobacterium that is adaptable to a widerange of environments including deserts, where it can maintain viability after desiccation. This species is also capable of photoautotrophic growth under far-red light (FRL, 750 nm) The proteomic comparison in ...
ORGANISM(S): Chroococcidiopsis thermalis PCC 7203 
2022-03-01 | PXD018129 | Pride
Chroococcidiopsis thermalis Phycobilisome Thylkoid Photosystem 
ProteomicsDeep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS
This study demonstrates how the latest UHPLC (ultra-high-performance liquid chromatography) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with nano-flow liquid c...
ORGANISM(S): Homo sapiens (Human) 
2022-09-12 | PXD035665 | Pride
Human Lcms Cells Orbitrap 
ProteomicsOptimization of data-independent acquisition-MS for deep proteome analysis
Data-independent acquisition (DIA)-MS analysis overtops the existing data-dependent acquisition (DDA)-MS analysis enabling deep proteome coverage and precise relative quantitative analysis in single-shot LC-MS/MS. We constructed a single-shot LC-MS/MS system with an MS measurement time of 90 min for...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms Mus Musculus (mouse) 
2019-10-28 | PXD016032 | JPOST Repository
UnknownOptimization of Data-Independent Acquisition Mass Spectrometry for Deep and Highly Sensitive Proteomic Analysis.
Not available
S-EPMC6928715 | biostudies-literature
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