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Chromatin immunoprecipitation (ChIP) was performed in biological replicates. For H3K27me3 ChIP assays, cells were cross-linked with 1% (vol/vol) formaldehyde. For SUZ12 and EZH2 ChIP assays, cells were cross-linked with 2 mM disuccinimidyl glutarate (DSG) and 1% (vol/vol) formaldehyde.
ORGANISM(S): Mus musculus 
2024-12-31 | GSE213014 | GEO
Performing ChIP-seq analyses in clinical specimens has remained largely challenging due to multiple technical limitations and low quantities of starting material, resulting in low enrichments and poor signal-to-noise ratio. Here, we refined the original protocols for transcription factor ChIP-seq an...
ORGANISM(S): Homo sapiens 
2018-12-27 | GSE114737 | GEO
Mouse embryonic fibroblasts (MEFs) with doxycycline (Dox)-inducible reprogramming cassette MKOS-ires-mOrange and a Nanog-GFP reporter were transduced with lentiviral Dox-inducible Ty1-BFP (blue fluoresce protein with Ty1 tag in the N-terminus) or Hic2-Ty1 (Hic2 with Ty1 tag in the C-terminus) expres...
ORGANISM(S): Mus musculus 
ChIP-seq studies of Th17 cells in Malat1 KO and WT mice
An unresolved molecular paradox is how the glucocorticoid receptor (GR) activates some genes while potently repressing others. We carried out genome-wide localization and expression profiling experiments in primary bone marrow-derived mouse macrophages treated with Dexamethasone in the presence or a...
ORGANISM(S): Mus musculus 
ChIP-seq data from mouse liver over-expressing GFP and fed a PTU diet and treated with saline, or overexpressing biotinylated TRb1 and GFP and fed a PTU diet, treated with either saline or T3 Mouse liver was infected with adenovirus that either expressed only GFP or GFP and biotinylated TRb1 and wer...
ORGANISM(S): Mus musculus 
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