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GenomicsGlucocorticoid Receptor and Androgen Receptor DNA binding in LREX'
LREX' are a LnCAP/AR subline with natural expresison of the glucocorticoid receptor. We used the model to compare the AR and GR cistromes in prostate tissue. We determined the GR cistrone in the presence of Dex (100nM) treatment and the AR cistrome in the presene of DHT (1nM) treatment.
ORGANISM(S): Homo sapiens 
2013-12-27 | E-GEOD-51497 | biostudies-arrayexpress
GenomicsGlucocorticoid receptor profiling in U2OS cells expressing GRalpha or GRgamma isoform
To determine whether insertion of a single amino acid had an effect on Glucocorticoid receptor (GR) occupancy, we performed ChIP-seq of GRalpha and GRgamma in the same U2OS osteosarcoma cell lines, upon glucocorticoid treatment. These assays are also part of submission E-MTAB-2955
ORGANISM(S): Homo sapiens 
2014-08-08 | E-MTAB-2731 | biostudies-arrayexpress
GenomicsLigand-dependent genomic function of glucocorticoid receptor in triple-negative breast cancer
Glucocorticoids (GC) have been widely used as coadjuvants in the treatment of solid tumors, but GC treatment may be associated with poor pharmacotherapeutic response and/or prognosis. The genomic action of GC in these tumors is largely unknown. Here we find that dexamethasone (Dex, a synthetic GC) r...
ORGANISM(S): Homo sapiens 
2015-09-18 | E-GEOD-56022 | biostudies-arrayexpress
TranscriptomicsHNF4A is required to specify glucocorticoid action in the liver - RNAseq
The glucocorticoid receptor (GR) is a nuclear hormone receptor critical to the regulation of energy metabolism and the inflammatory response. The actions of GR have been shown to be highly dependent on context. Here, we demonstrate the necessity for liver lineage-determining factor hepatocyte nuclea...
ORGANISM(S): Mus musculus 
2021-04-09 | E-MTAB-10247 | biostudies-arrayexpress
ProteomicsQuantitative Proteomics Analysis of Membrane Glucocorticoid Receptor Activation in Rainbow Trout Skeletal Muscle
In the present work, we evaluated the effects of membrane-initiated cortisol actions in vivo in the proteome of rainbow trout (Oncorhynchus mykiss) skeletal muscle. Quantitative iTRAQ analyses were performed to examine proteomic changes in rainbow trout stimulated with physiological concentrations o...
ORGANISM(S): Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) 
2019-10-30 | PXD015383 | Pride
Rainbow trout  skeletal muscle  membrane glucocorticoid receptor 
GenomicsGlucocorticoid receptor profiling in IMR90,K562 and U2OS (ChIP-seq)
To identify the sequences responsible for recruitment of Glucocorticoid receptor (GR) to individual loci, we performed ChIP-seq and ChIP-exo that combines chromatin immunoprecipitation with an exonuclease digestion step. We performed these experiments in three cell lines : IMR90 (ATTC:CCL-186), U2O...
ORGANISM(S): Homo sapiens 
2015-02-25 | E-MTAB-2955 | biostudies-arrayexpress
GenomicsGenome wide binding of glucocorticoid receptor in dexamethasone treated mouse bone marrow derived macrophages
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Mus musculus 
2015-12-17 | E-GEOD-61877 | biostudies-arrayexpress
GenomicsGenome wide binding of glucocorticoid receptor in dexamethasone treated human monocyte derived macrophages
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Homo sapiens 
2015-12-17 | E-GEOD-61878 | biostudies-arrayexpress
TranscriptomicsHes1 and Glucocorticoid Signaling in Bone Sarcoma Cells
To investigate the role of HES1 during glucocorticoid signaling in bone sarcoma cells (U2OS-GR cells) , we employed whole-genome microarray expressions in mRNA extracted from control U2OS-GR and Hes1-overexpressed U2OS-GR cells. RNA isolated from control U2OS-GR and Hes1-overexpressed U2OS-GR cells...
ORGANISM(S): Homo sapiens 
2014-01-21 | E-GEOD-52727 | biostudies-arrayexpress
TranscriptomicsGlobal Analysis of Glucocorticoid Receptor-Associated Transcripts and Identification of a Target RNA Motif
Posttranscriptional regulation is emerging as a key factor in glucocorticoid (GC)-mediated gene regulation. We investigated the role of the human glucocorticoid receptor (GR) as an RNA-binding protein and its effect on mRNA turnover in human airway epithelial cells. Cell treatment with the potent ...
ORGANISM(S): Homo sapiens 
2011-06-24 | E-GEOD-24748 | biostudies-arrayexpress
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