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ProteomicsA microsomal proteomics view of H2O2- and ABA-dependent responses
The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated NADPH oxidases. Although quantitative proteomics studie...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-08-22 | PXD006513 | Pride
Hydrogen peroxide (h2o2)  abscisic acid (aba)  microsomal proteomics  quantitative proteomics  mass spectrometry 
ProteomicsSimple Strategy of Integrating the Micro-centrifugation-based Extraction and 4-Hexylphenylazosulfonate for the Preparation of Plant Microsomal Proteins for Bottom-Up Proteomics
The preparation of high-quality microsomal proteins (MPs) is of critical importance to high-throughput microsomal proteomics. The isolation of MPs for proteomic analysis has to date relied on Ultracentrifugation-based methods (UBMs), however, these methods are labor-intensive, time-consuming, and un...
ORGANISM(S): Oryza sativa subsp. japonica (Rice) 
2021-09-29 | PXD025132 | Pride
Rice leaves Perseus Label-free quantitative proteomics Ultracentrifugation Micro-centrifugation Azo Maxquant Microsomal membrane extraction Plasma membrane Sds 
UnknownA Microsomal Proteomics View of H₂O₂- and ABA-Dependent Responses.
Not available
S-EPMC5620539 | biostudies-literature
ProteomicsRegulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol‐4‐kinases β1 and β2 in Arabidopsis thaliana
Phosphatidylinositol-4-kinases β1 and β2 (PI4Kβ1/PI4Kβ2), which are responsible for phosphorylation of phosphoinositol to phosphatidylinositol-4-phosphate, have an important role in vesicular trafficking in plants. Moreover, PI4Kβ1/PI4β2 negatively regulate biosynthesis of phytohormone salicylic aci...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-24 | PXD019737 | Pride
Arabidopsis thaliana Microsomal fraction Phosphatidylinositol-4-kinases β1 and β2 Salicylic acid Label-free quantification Lc-ms/ms 
ProteomicsQuantitative LC-MS/MS Proteomics of microsomal fractions from adult human livers
We report label-free and targeted quantification of xenobiotic metabolizing enzymes (XME) and transporters in 39 human liver microsomal samples. More than 2800 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models for dosage regimen design a...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD021025 | Pride
Human Adult livers Quantitative proteomics Lc-ms/ms 
UnknownAn Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics.
Not available
S-EPMC8460067 | biostudies-literature
ProteomicsConsequences of Cyp2c44 deletion on proteome of the microsomal fraction of primary polyoma middle T (PyMT) tumors
Here we compared the proteomes of microsomal fractions from primary tumors isolated from PyMT mice versus PyMTxCyp2c44-/- mice.
ORGANISM(S): Mus musculus (Mouse) 
2020-03-03 | PXD017303 | Pride
Breast cancer Macrophage Wdfy1 Lymphangiogenesis Lc-msms Metastasis 
UnknownQuantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry.
Not available
S-EPMC1444949 | biostudies-literature
ProteomicsEmbryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity
Somatic embryogenesis is an important biological process in several plant species, including sugarcane. Proteomics approaches have shown that H+ pumps are differentially regulated during somatic embryogenesis; however, the relationship between H+ flux and embryogenic competence is still unclear. Thi...
ORGANISM(S): Saccharum officinarum 
2018-06-26 | PXD009858 | Pride
Somatic embryogenesis  ion-selective vibrating probe  saccharum ssp.  h+ pumps  microsomal proteomics 
UnknownProteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers.
Not available
S-EPMC5372229 | biostudies-literature
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