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TranscriptomicsBRAF Mutation, NRAS Mutation, and the Absence of an Immune-Related Expressed Gene Profile Predict Poor Outcome in Patients with Stage III Melanoma
Prediction of outcome for melanoma patients with surgically-resected macroscopic nodal metastases is very imprecise. We performed a comprehensive clinicopathologic assessment of fresh-frozen macroscopic nodal metastases and the preceding primary melanoma, somatic mutation profiling and gene expressi...
ORGANISM(S): Homo sapiens 
2013-12-10 | E-GEOD-53118 | biostudies-arrayexpress
ProteomicsDynamic conformational equilibria in the active states of KRas and NRas
The design of potent RAS inhibitors benefits from a molecular understanding of the dynamics in KRAS and NRAS and their oncogenic mutants. Here we characterize switch-1 dynamics in GTP-state KRAS and NRAS by 31P NMR, by 15N relaxation dispersion NMR, hydrogen-deuterium exchange mass spectrometry (HDX...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054924 | Pride
Ras inhibitors  nras  kras  hdx-ms  hydrogen/deuterium exchange mass spectrometry  oncogenic mutants 
GenomicsB11 cells created by replacing the NRAS-G12D mutation in THP1 cells with a dox-inducible NRAS-G12V mutation
B11 cells created by replacing the NRAS-G12D mutation in THP1 cells with a dox-inducible NRAS-G12V mutation
PRJNA476487 | ENA
GenomicsEffect of DNMT3A R882H mutation or WT expression on epigenetic landscapes of hematopoietic stem/progenitor cells with NRAS G12D co-transduction (ChIP-seq)
DNA Methyltransferase 3A (DNMT3A) is frequently mutated in various hematopoietic malignancies; however, the underlying oncogenic mechanisms remain elusive. Here, we report that DNMT3A mutational ‘hotspot’ at Arg882 (DNMT3A-R882H) cooperates with constitutively activated RAS in transforming murin...
ORGANISM(S): Mus musculus 
2016-06-23 | E-GEOD-71472 | biostudies-arrayexpress
GenomicsEffect of DNMT3A R882H mutation or WT expression on global DNA methylation patterns of hematopoietic stem/progenitor cells with NRAS G12D co-transduction (eRRBS)
DNA Methyltransferase 3A (DNMT3A) is frequently mutated in various hematopoietic malignancies; however, the underlying oncogenic mechanisms remain elusive. Here, we report that DNMT3A mutational ‘hotspot’ at Arg882 (DNMT3A-R882H) cooperates with constitutively activated RAS in transforming murin...
ORGANISM(S): Mus musculus 
2016-06-23 | E-GEOD-71473 | biostudies-arrayexpress
TranscriptomicsEffect of DNMT3A R882H mutation or WT on gene expression in hematopoietic stem/progenitor cells with NRAS G12D co-transduction (Microarray)
DNA Methyltransferase 3A (DNMT3A) is frequently mutated in various hematopoietic malignancies; however, the underlying oncogenic mechanisms remain elusive. Here, we report that DNMT3A mutational â??hotspotâ?? at Arg882 (i.e., DNMT3A-R882H) cooperates with constitutively activated RAS in transforming...
ORGANISM(S): Mus musculus 
2016-06-23 | E-GEOD-71437 | biostudies-arrayexpress
TranscriptomicsB11 cells created by replacing the NRAS-G12D mutation in THP1 cells with a dox-inducible NRAS-G12V mutation
To study the role of NRAS mutations in cell proliferation and self-renewal in acute myeloid leukemia (AML), the human AML cell line, THP1, was modified to replace its naturally occurring heterozygous NRAS-G12D mutation with a doxycycline(dox)-inducible heterozygous NRAS-G12V mutation. The endogenous...
ORGANISM(S): Homo sapiens 
2022-07-25 | GSE115911 | GEO
TranscriptomicsOncogenic Nras has a bimodal effect on hematopoietic stem cells promoting proliferation and self-renewal
Pre-leukemic mutations are thought to promote clonal expansion of hematopoietic stem cells (HSCs) by increasing self-renewal and competitiveness. However, mutations that increase HSC proliferation tend to reduce competitiveness and self-renewal potential, raising the question of how a mutant HSC can...
ORGANISM(S): Mus musculus 
2014-03-14 | E-GEOD-45194 | biostudies-arrayexpress
TranscriptomicsOncogenic potential of RAS isoforms in melanocytes
We compared the transformation efficiencies of mutant NRAS and KRAS in immortal, non-transformed Ink4a/Arf-deficient melanocytes. NRAS mutation leads to increased cellular proliferation and is potently tumorigenic. In contrast, KRAS mutation does not enhance melanocyte proliferation and is only weak...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-6194 | biostudies-arrayexpress
TranscriptomicsWild-Type Nras Lacks Tumor Suppressor Activity and Nras Oncogene Dosage Strongly Modulates Hematopoietic Transformation
Contemporary treatment of pediatric acute myeloid leukemia (AML) requires the assignment of patients to specific risk groups. To explore whether expression profiling of leukemic blasts could accurately distinguish between the known risk groups of AML, we analyzed 130 pediatric and 20 adult AML diagn...
ORGANISM(S): Homo sapiens 
2013-07-02 | E-GEOD-43176 | biostudies-arrayexpress
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