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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsFunctional effects of CYP3A-CYP2E1 interactions and their role in activation of CYP3A enzymes by chronic alcohol exposure
Aiming to examine the effect of chronic alcohol exposure on the activity of CYP3A enzymes in human liver, we studied the metabolism of two CYP3A-specific substrates, 7-benzyloxyquinoline (7-BQ) and ivermectin, in 23 preparations of human liver microsomes (HLM) obtained from donors with documented al...
ORGANISM(S): Homo sapiens (Human) 
2026-09-18 | PXD079247 | Pride
Cytochrome p450  cyp2e1  cyp3a4  cyp3a5  nadph-cytochrome p450 reductase  pro-tein-protein interactions  7-benzyloxyquinoline  ivermectin  human liver microsomes  alcohol consumption  alcohol-drug interactions  cross-linking mass spectrometry 
ProteomicsAssay for characterizing adsorption-properties of surfaces (APS)
Analytes, from sample preparation, until entering an analytical instrument, are prone to adsorb to surfaces, driven by the chemical properties of the surface and the liquids they are dissolved in. This problem can be addressed with internal standards when a single or few known analytes are quantifie...
ORGANISM(S): Homo sapiens (Human) 
2024-08-30 | PXD044268 | Pride
Adsorption Sample loss Surfaces Label-free quantification Lc-ms/ms 
ProteomicsGlobal proteome-wide analysis of cysteine S-nitrosylation in Toxoplasma gondii
Toxoplasma gondii transmitted via various route and rapidly duplicated during the acute infection, which caused the important zoonosis regarding to medicine and veterinary, toxoplasmosis. T. gondii possessed innate capability of producing nitric oxide (NO) and nitric oxide derivatives and S-nitrosyl...
ORGANISM(S): Toxoplasma gondii 
2024-01-26 | PXD046083 | Pride
Toxoplasma gondii  post-translational modifications  cysteine s-nitrosylation  s-nitrosylated pro-teome 
ProteomicsComparative proteomic analysis between female and hermaphrodite flower buds of papaya (Carica papaya)
Papaya (Carica papaya) is a trioecious species, with female, male, and hermaphrodite plants. Because of sex segregation, selecting hermaphroditic plants is vital for orchard establishment due to their higher commercial value. In addition to the costly sexing step, environmental stresses can result i...
ORGANISM(S): Carica papaya 
2024-06-23 | PXD050497 | Pride
Sexual differentiation  floral development  proteome  phenylpropanoid  sporopollenin biosynthesis. 
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