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Proteomics,MultiomicsProtrusion vs Cell-body SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-10 | MSV000079570 | MassIVE
ProteomicsProtrusion vs Cell-body pulsed-SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. Apart from proteins, several mRNAs are known to be localised to cell protrusions but to what extent RNA localisation and local translation contributes toward front-back assymetry is unknown...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2015-10-09 | PXD002649 | Pride
Pulsed-silac  local translation  protrusion 
ProteomicsProtrusion vs Cell-body SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo sapiens (Human) 
2015-10-09 | PXD000914 | Pride
Human silac proteomics protrusion lc-ms/ms orbitrap-velos maxquant 
TranscriptomicsQUANTSEQ 3' sequencing of protrusions and cell bodies of control (NT) or LARP6 siRNA (LARP6 KD) transfected MDA-MB231 breast cancer cells
To assess how LARP6 affects mRNA localisation to cell-protrusions, we transfected MDA-MB231 cells with either non-targeting control siRNA or LARP6 siRNA for 72 hrs, before fractionating the cells into protrusion and cell-bodies, and analysed total RNA from each fraction via Lexogen QUANTSEQ FWD 3' m...
ORGANISM(S): Homo sapiens 
2020-10-30 | E-MTAB-9520 | biostudies-arrayexpress
UnknownMorphodynamic profiling of protrusion phenotypes.
Not available
S-EPMC1367294 | biostudies-literature
UnknownProtrusion fluctuations direct cell motion.
Not available
S-EPMC4119278 | biostudies-literature
UnknownEndothelial Surface Protrusion by a Point Force.
Not available
S-EPMC4788716 | biostudies-literature
ProteomicsAssessing the short and long-term impacts of protrusion induction on the proteome of MDA-MB231 cells
To assess the impact of protrusion induction on the proteome of mesenchymal-like migratory cells, MDA-MB231 breast cancer cells were seeded on top of collagen-I coated 3 micron transwell filters (corning). No media was added to the bottom chamber and cells were allowed to adhere to filter overnight....
ORGANISM(S): Homo sapiens (Human) 
2020-10-20 | PXD021206 | Pride
Tmt  protrusion  mda-mb231  orbitrap 
GenomicsProtrusion vs cell-body mRNA analysis by RNA-seq
Protrusion vs cell-body mRNA analysis by RNA-seq
PRJEB6243 | ENA
UnknownProtrusion growth driven by myosin-generated force.
Not available
S-EPMC9940483 | biostudies-literature
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