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A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50M-NM-
ORGANISM(S): Mus musculus 
A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 106 CFU of Streptococcus pneumoniae serotype 3 into the left lung lobe. Mice were euthanized after 15 hours after instillation, and left lung lobes were collecte...
ORGANISM(S): Mus musculus 
In this experiment, we carried out a global analysis of specific RNAs that are bound to the RelA protein. CLIP-Seq experiment was carried out with E. coli RelA wild type and RelA::C289Y mutant strains. Libraries were prepared and high throughput sequencing was done.
ORGANISM(S): Escherichia coli K-12 
Background: Lymphotoxin signaling via the lymphotoxin-β receptor (LTβR) has been implicated in several biological processes, ranging from development of secondary lymphoid organs, maintenance of splenic tissue, host defense against pathogens, autoimmunity, and lipid homeostasis. The major transcript...
ORGANISM(S): Mus musculus 
This experiment tests the gene expression of the RelA mutant (ORF03691/GSU2236) versus wild type G. sulfurreducens during stationary phase growth. Batch culture, Acetate electron donor, Fumarate electron acceptor.
ORGANISM(S): Geobacter sulfurreducens 
Effect of ppGpp on transcript level after RelA overexpression (45 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
CLIP-Seq experiment of Escherichia coli RelA wild type and RelA::C289Y mutant strains to identify specific RNAs that bind RelA
Effect of ppGpp on transcript level after RelA overexpression (90 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
Reconstituted Rela-/- MEF
WT and mutant strains (relA-, relA-spoT-) were submitted to starvation for isoleucine. 40 minutes after starvation, RNA was extracted. All samples were compared to RNA from rapidly growing WT cells in identical medium.

A data file with additional processed data is available for download fr...
ORGANISM(S): Escherichia coli 
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