Sort   by:  
 Page size 
ProteomicsProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS: Preparation of more than 4000 native protein maps
Soluble proteins of human bronchial smooth muscle cells (HBSMC) were separated by nondenaturing micro 2DE and a 30 mm X 40 mm area of the CBB-stained slab gel (1.0 mm thick) was cut into 1.1 mm X 1.1 mm squares, then the proteins in the 972 gel pieces (squares) were applied to quantitative LC-MS/MS....
ORGANISM(S): Homo sapiens (Human) 
2015-08-10 | PXD001471 | Pride
Cellular proteins Native protein map Nondenaturing micro 2de Lc-ms/ms Quantitative protein-protein interaction 
ProteomicsGlobal mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a representative gel was selected, on which a total of 136 CBB-stained spots were numbered and subjected to in-gel digestion and label-free quantitative LC-MSE (MS/MS in data-independent acquisition mode). The analysi...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-10-14 | PXD004395 | Pride
Native proteomics Adult male rat plasma proteome Nondenaturing micro 2de Global mapping Label-free quantitative lc-mse 
UnknownProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 1. Preparation of more than 4000 native protein maps.
Not available
S-EPMC6084315 | biostudies-literature
ProteomicsProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS: Preparation of more than 4000 native protein maps
Soluble proteins of human bronchial smooth muscle cells (HBSMC) were separated by nondenaturing micro 2DE and a 30 mm X 40 mm area of the CBB-stained slab gel (1.0 mm thick) was cut into 1.1 mm X 1.1 mm squares, then the proteins in the 972 gel pieces (squares) were applied to quantitative LC-MS/MS....
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-23 | MSV000080002 | MassIVE
TranscriptomicsMolecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells
FLT3 is the most frequently mutated gene in AML – up to 40% of AML harbor an activating mutation within FLT3 gene. Though AML is a relatively rare disease, such a high mutability rate, as observed with FLT3 gene, is striking. To elucidate the molecular background of this phenomenon, we have establ...
ORGANISM(S): Mus musculus 
2010-04-08 | E-GEOD-12766 | biostudies-arrayexpress
Proteomics2DE images and LC-MS/MS of smooth and rough strains of Salmonella Gallinarum
Antigens of SG002, field isolated Salmonella Gallinarum in Korea, were tested against antiserum of SG002 and SR2-N6 (rough mutant of SG002) using 2DE western blotting. Different blots obtained were analyzed using LC-MS/MS. Outer membrane protein extracts of SR2-N6 were also tested against the antise...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Gallinarum str. 9184 
2020-10-20 | PXD022024 | Pride
Antibody Outer membrane protein Salmonella gallinarum 
UnknownProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 2. Similarity search between protein maps for the analysis of protein complexes.
Not available
S-EPMC5157777 | biostudies-literature
ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
ProteomicsUpdate on virtual-experimental 2DE approach in chromosome-centric human proteome project
In the boundaries of chromosome-centric Human Proteome Project c-HPP to obtain information about proteoforms coded by chromosome 18, several cell lines were analyzed. In our study we have been using proteoform separation by 2DE (sectional analysis) and semi-virtual 2DE with following shotgun mass-sp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-12 | MSV000086448 | MassIVE
UnknownPrincipal component analysis on LC‑MS/MS and 2DE‑MALDI‑TOF in glioblastoma cell lines reveals that mitochondria act as organelle sensors of the metabolic state in glioblastoma.
Not available
S-EPMC7336416 | biostudies-literature
Sort   by:  
 Page size