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The 4T1 cells were labeled with BxxP via HA-PCN nanozyme as described above. After washing 5 times with PBS, cells were collected and lysed in RIPA buffer containing 1 % (v/v) Protease inhibitor cocktail (ThermoFisher Scientific) and 1 % (v/v) PMSF under bath-sonication, followed by centrifugation f...
ORGANISM(S): Mus musculus (Mouse) 
2025-07-29 | PXD057009 | Pride
After establishing a subcutaneous 4T1 tumor model in mice, we conducted PATCH treatment using HA-PCN. Tumors were harvested post-treatment for single-cell RNA sequencing to analyze cellular and transcriptional changes within the tumor microenvironment.
ORGANISM(S): Mus musculus 
2025-08-04 | GSE304370 | GEO
PATCH treatment in vivo
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