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TranscriptomicsTranscription profiling of Drosphila samples to explain differences in saturation levels for affymetrix genechip arrays
The experimental spike-in studies of microarray hybridization conducted by Affymetrix demonstrate a nonlinear response of fluorescence intensity signal to target concentration. It was shown that the Langmuir adsorption isotherm recapitulates a general trend of signal response to concentration. Howev...
ORGANISM(S): Drosophila melanogaster 
2008-06-15 | E-GEOD-7110 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Drosophila overexpressing MnSOD
Profiled the transcriptional response to over-expression Manganese-SOD (MnSOD) in adult Drosophila. A doxycycline-regulated system was employed to induce MnSOD over-expression, resulting in mean and maximal lifespan increases of ~ 20%. Examination of the genome-wide response to this lifespan inter...
ORGANISM(S): Drosophila melanogaster 
2008-06-15 | E-GEOD-7159 | biostudies-arrayexpress
TranscriptomicsTranscription Profiling of Formalin-Fixed Paraffin-Embedded Samples and fresh frozen samples by Affymetrix Microarrays as a qualitative assessment
Background: To date, few studies have systematically characterized microarray gene expression signal performance with degraded RNA from formalin-fixed paraffin-embedded (FFPE) specimens in comparison to intact RNA from unfixed fresh-frozen (FF) specimens. Methodology: RNA was extracted and isolated ...
ORGANISM(S): Homo sapiens 
2010-11-25 | E-GEOD-19249 | biostudies-arrayexpress
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