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ProteomicsCrosslinking mass spectrometry of the p300 catalytic core complexed with the nucleosome
p300 is a histone acetyltransferase that associates with crucial biological processes. p300 acetylates all four histones in the nucleosome, a basic unit of chromatin, and alters chromatin structure and dynamics. In this study, we performed structural and biochemical analysis to understand the nucleo...
ORGANISM(S): Homo Sapiens (human) 
2022-06-29 | PXD033804 | JPOST Repository
OtherData for: Short-chain fatty acids activate acetyltransferase p300/CBP
The short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced to changes in epigenetic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-12 | MSV000087800 | MassIVE
TranscriptomicsTranscription profiling of mouse C2AS12 cells infected with (1) wt p300 + tTA, (2) p300deltaTAZ2 +tTA, (3) wt p300 +tTA +doxycycline to define the transcriptional network regulated by ectopic expression of the transcriptional co-activator protein, p300 and define its role in promoting muscle cell survival
The primary focus of these studies is to define the transcriptional network regulated by ectopic expression of the transcriptional co-activator protein, p300, in order to define its mechanism(s) of action in promoting muscle cell survival. Our laboratory has generated a murine C2-derived myoblast ce...
ORGANISM(S): Mus musculus 
2007-12-07 | E-GEOD-4224 | biostudies-arrayexpress
TranscriptomicsInhibition of p300 Lysine Acetyltransferase activity by Luteolin reduces tumor growth in head and neck squamous cell carcinoma (HNSCC) xenograft mouse model [mRNA]
Chromatin acetylation is attributed with distinct functional relevance with respect to gene expression in normal and diseased conditions thereby leading to a topical interest in the concept of epigenetic modulators and therapy. We report here the identification and characterization of the acetylatio...
ORGANISM(S): Homo sapiens 
2016-07-05 | E-GEOD-75029 | biostudies-arrayexpress
ProteomicsTime-resolved analysis reveals rapid dynamics and broad scope of the CBP/p300 acetylome
The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators; however, their acetylation targets, site-specific acetylation kinetics, and function in proteome regulation are incompletely understood. We combined quantitative proteomics with novel CBP/p300-specific catalytic i...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
MSV000095981 | MassIVE
GenomicsDUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes
Ectopic expression of the double homeodomain transcription factor DUX4 causes facioscapulohumeral muscular dystrophy (FSHD). Mechanisms of action of DUX4 are currently unknown. Using immortalized human myoblasts with a titratable DUX4 transgene, we identify by mass spectrometry an interaction betwee...
ORGANISM(S): Homo sapiens 
2016-03-10 | E-GEOD-78158 | biostudies-arrayexpress
TranscriptomicsTwo Histone/Protein Acetyltransferases, CBP and p300, Are Indispensable for Foxp3+ T-regulatory Cell Development and Function
T-regulatory (Treg) cells are important to immune homeostasis, and Treg cell deficiency or dysfunction leads to autoimmune disease. An histone/protein acetyltransferase (HAT), p300, was recently found important for Treg function and stability, but further insights into the mechanisms by which p300 o...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-60318 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse splenic B cells expressing a dominant-negative p300 allele which lacks histone acetyltransferase activity
The p300 protein is one of more than 15 known mammalian HATs. We have used mice that carry a mutant allele of the p300 gene, which encodes a full-length protein that, however, lacks detectable acetyltransferase (AT)-activity and that acts in a dominant-negative manner. In addition, the allele is con...
ORGANISM(S): Mus musculus 
2006-07-01 | E-MEXP-747 | biostudies-arrayexpress
ProteomicsTargeted degradation of the enhancer lysine acetyltransferases CBP and p300
The enhancer factors CBP/p300 maintain gene expression programs through lysine acetylation of chromatin and transcriptional regulators and by scaffolding functions mediated by several protein-protein interaction domains. We have designed dCBP-1, a compound that induces degradation of CBP/p300, and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-15 | MSV000086457 | MassIVE
ProteomicsNuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity
The transcriptional co-activator and acetyltransferase p300 is required for fundamental cellular processes, including differentiation and growth. Here, we report that p300 forms phase separated condensates in the cell nucleus. The phase separation ability of p300 is regulated by autoacetylation and ...
ORGANISM(S): Homo sapiens (Human) 
2021-08-26 | PXD026898 | Pride
P300 nuclear condensates hat activity 
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