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TranscriptomicsHCT116 cells isogenic for p53 expression upon treatment with actinomycin D and nutlin-3
HCT116 wild type cells express p53. The isogenic HCT116 p53KO cells have the p53 gene knocked out. Cells were treated for 16 hours. Dosages for actinomycin D treatment was 1 nM, 10 nM, and 100 nM. Dosage for nutlin-3 was 4 uM. Keywords: dose response Measuring the gene expression profile of the is...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-12459 | biostudies-arrayexpress
TranscriptomicsLow-dose actinomycin D preferentially disrupts EWS-FLI1DNA binding.
Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds that disrupt the binding of EWS-FLI1 to its cognate DNA targets. A number of DNA...
ORGANISM(S): Homo sapiens 
2013-08-26 | E-GEOD-45414 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of ire1 mutants after treatment with or without tunicamycin in the presence of actinomycin D
Arabidopsis seedlings of wildtype or ire1a ire1b double mutant were treated with or without tunicamycine in the presence of actinomycin D (ActD). 12 samples
ORGANISM(S): Arabidopsis thaliana 
2013-03-19 | E-GEOD-39690 | biostudies-arrayexpress
TranscriptomicsRNA half-life analysis after transcriptional blocking with actinomycin D
Transcriptional profiling of HeLa cells treated with actinomycin D for increasing amounts of time. Relative abundance to control cells was used to estimate the half-life of lncRNAs. Two-condition experiment, actinomycin D-treated vs. untreatted HeLa cells. Biological replicates: 2. Technical replic...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-62963 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast using samples prepared with actinomycin D to exclude antisense artifacts
Recent transcription profiling studies have revealed an unanticipatedly large proportion of antisense transcription across eukaryotic and bacterial genomes. However, the extent and significance of antisense transcripts is controversial partly because experimental artifacts are suspected. Here, we pr...
ORGANISM(S): Saccharomyces cerevisiae 
2007-10-30 | E-TABM-361 | biostudies-arrayexpress
TranscriptomicsExpression data from treatment of actinomycin D and triptolide on MCF7 cells
Expression data from treatment of actinomycin D (2.5uM) and triptolide (500 nM) on MCF7 cells for 2, 4 and 6 hours. We used microarrays to explore the mechanism of triptolide action. MCF7 cells were treated with actinomycin D or triptolide for 2, 4 and 6 hours, RNA was extracted and hybridization on...
ORGANISM(S): Homo sapiens 
2011-10-17 | E-GEOD-28662 | biostudies-arrayexpress
TranscriptomicsActinomycin D-induced mitochondrial stress targets PML during NPM1c-AML therapy
Microarrays transcriptomic data analyses from acute myeloid leukemia cell line OCI-AML2 and primary acute myeloid leukemia cells treated with actinomycin D.
ORGANISM(S): Homo sapiens 
2021-05-10 | E-MTAB-10397 | biostudies-arrayexpress
TranscriptomicsTranscription profiling time series of Sulfolobus acidocaldarius and Sulfolobus solfataricus after treatment with actinomycin D
Transcription inhibition by actinomycin D. RNA samples extracted at 3, 6, 9, 12, 15 min post actinomycin D. Each cDNA hybridised against reference cDNA from untreated culture.
ORGANISM(S): Sulfolobus solfataricus 
2006-11-08 | E-MEXP-894 | biostudies-arrayexpress
TranscriptomicsExpression data from CCRF_CEM cell line treated with 7-(2-Thienyl)-7-deazaadenosine (AB61), tubercidin and actinomycin D
CCRF_CEM cell line was treated by AB61 which is potent cytotoxic compound, as the positive controls were used tubercidin and actinomycin D. To assay the biological activity of the AB61 more broadly we performed transcriptional microarray analysis. . CCRF_CEM cell line was treated by AB61, tubercidin...
ORGANISM(S): Homo sapiens 
2014-10-22 | E-GEOD-62593 | biostudies-arrayexpress
TranscriptomicsEffect of prolactin and actinomycin D on gene expression in murine AML12 and Hepa 1-6 hepatocytes
Transcriptional profiling of murine hepatocyte gene expression following exposure to prolactin or actinomycin D Prolactin or actinomycin D-treated versus serum-free control cells: AML12 and Hepa 1-6. Biological replicates: 2 per cell line per treatment protocol.
ORGANISM(S): Mus musculus 
2014-04-25 | E-GEOD-57072 | biostudies-arrayexpress
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