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GenomicsGenome-wide Chromatin Maps Derived from Limited Numbers of Hematopoietic Progenitors
Whole genome mapping of protein-DNA interactions can be performed by coupling chromatin immunoprecipitation (ChIP) with high-throughput sequencing (ChIP-Seq). However, current methods require large amounts of starting materials which precludes their application to rare cell types. Here, we combine a...
ORGANISM(S): Mus musculus 
2010-07-01 | E-GEOD-22075 | biostudies-arrayexpress
GenomicsGenome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-se...
ORGANISM(S): Homo sapiens 
2014-05-18 | E-GEOD-55887 | biostudies-arrayexpress
GenomicsASXL1 Mutations Promote Myeloid Transformation Through Loss of PRC2-Mediated Gene Repression
Recurrent somatic ASXL1 mutations occur in patients with myelodysplasia (MDS), myeloproliferative neoplasms (MPN), and acute myeloid leukemia (AML), and are associated with adverse outcome. Despite the genetic and clinical data implicating ASXL1 mutations in myeloid malignancies, the mechanisms of t...
ORGANISM(S): Homo sapiens 
2012-08-21 | E-GEOD-38861 | biostudies-arrayexpress
GenomicsDegree of Recruitment of DOT1L to MLL-AF9 Defines Level of H3K79 Di- and Tri-methylation on Target Genes and Transformation Potential
The MLL gene is a common target of chromosomal translocations found in human leukemia. MLL-fusion leukemias are consistently poor prognosis. One of the most common translocation partners is AF9 (a.k.a. MLLT3). MLL-AF9 recruits DOT1L, a histone 3 lysine 79 methyltransferase (H3K79me1/me2/me3), leadin...
ORGANISM(S): Mus musculus 
2015-04-30 | E-GEOD-64365 | biostudies-arrayexpress
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