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ProteomicsxIP-MS: topological analysis of chromatin-associated protein complexes using single affinity purification
In recent years, cross-linking mass spectrometry (XL-MS) has proven to be a robust and effective method of interrogating macromolecular protein complex topologies at peptide resolution. Traditionally, XL-MS workflows have utilized homogenous complexes obtained through time-limiting reconstitution, t...
ORGANISM(S): Homo sapiens (Human) 
2015-11-17 | PXD002987 | Pride
Affinity purification Chemical cross-linking mass-spectrometry Chromatin 
ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-17 | MSV000083252 | MassIVE
ProteomicsHighly accurate protein complex retrieval by affinity enrichment MS rather than affinity purification MS
Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identifi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-03-26 | PXD000955 | Pride
Ap-ms Ae-ms Yeast Interaction proteomics Label-free quantification 
ProteomicsIdentifying the interacting proteins of lignin biosynthetic P450 enzymes by affinity purification coupled with LC-MS/MS analysis
This project aims to identify the potential interacting proteins of lignin biosynthetic P450 enzymes by affinity purification and LC-MS/MS analysis. To this end, the genomic pieces of three lignin biosynthetic P450 genes C4H, C3’H and F5H were cloned into pMDCpC4H-HPB vector to generate HPB-fusion c...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-05-02 | PXD009033 | Pride
Lignin C3'h Affinity purification F5h C4h Lc-ms/ms 
ProteomicsIdentification of inositol 1,4,5-trisphosphate-binding proteins by heparin-agarose affinity purification and LTQ ORBITRAP MS in Oryza sativa
Inositol 1,4,5-trisphosohate (IP3) and its receptors play a pivotal role in calcium signal transduction in mammals. Some fractions of tonoplast and endoplasmic reticulum membranes have high affinity binding activity for IP3, and IP3 can induce Ca2+ release in several plants. However, no homologues o...
ORGANISM(S): Oryza sativa (Rice) 
2014-09-25 | PXD000763 | Pride
Oryza sativa Ip3 Ip3 binding protein Ltq orbitrap ms Heparin-agarose 
UnknownCross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification.
Not available
S-EPMC4813705 | biostudies-literature
UnknownAccurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).
Not available
S-EPMC4288248 | biostudies-literature
ProteomicsAPPLE-MS: A modular affinity purification-mass spectrometry method assisted by PafA- mediated proximity labeling
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-10-27 | PXD063145 | Pride
Ap-ms Proximity labeling Protein-protein interaction Interactome 
UnknownAffinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis.
Not available
S-EPMC8584136 | biostudies-literature
ProteomicsSEPT6 affinity purification-mass spectrometry (AP-MS)
Septin proteins interact intermolecularly to form hetero-oligomeric complexes that further assemble into higher-order filaments. To investigate whether the interactions among septin proteins are affected by RID, we conducted affinity purification-mass spectrometry (AP-MS) experiments. In this regard...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD046497 | Pride
Sept6 Rid Fatty-acylation 
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