Sort   by:  
 Page size 
TranscriptomicsElucidation of Transcriptome-Wide MicroRNA Binding Sites in Human Cardiac Tissues by Ago2 HITS-CLIP
We deciphered Ago2:RNA interactions in post-mortem human heart tissues using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across...
ORGANISM(S): Homo sapiens 
2016-08-09 | E-GEOD-83410 | biostudies-arrayexpress
TranscriptomicsRemodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates (part 3)
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Ago2/microRNA machinery has been shown to participate in stre...
ORGANISM(S): Homo sapiens 
2013-07-15 | E-GEOD-44378 | biostudies-arrayexpress
TranscriptomicsRemodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates (part 1)
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Ago2/microRNA machinery has been shown to participate in stre...
ORGANISM(S): Homo sapiens 
2013-07-15 | E-GEOD-44376 | biostudies-arrayexpress
TranscriptomicsRemodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates (part 2)
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Ago2/microRNA machinery has been shown to participate in stre...
ORGANISM(S): Homo sapiens 
2013-07-15 | E-GEOD-44377 | biostudies-arrayexpress
TranscriptomicsTranscriptome-wide discovery of microRNA binding sites in human brain by Ago2 HITS-CLIP [Ago2-miRNA-target mRNA complexes]
Here, seeking to gain insight into the array of transcripts engaged with miRNAs in human brain, we performed HITS-CLIP to profile transcriptome-wide Ago2:RNA interactions in a panel of eleven post-mortem adult human brain samples harvested from adult motor cortex and cingulate gyrus, regions associa...
ORGANISM(S): Homo sapiens 
2014-01-03 | E-GEOD-52082 | biostudies-arrayexpress
TranscriptomicsGenome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs
MicroRNAs (miRNAs) are a large family of 19-22nt non-coding RNAs that post-transcriptionally regulate their mRNA targets. Computational algorithms predict that over half of all genes are regulated by miRNAs, yet approaches for experimental identification of miRNA binding sites are now emerging. To ...
ORGANISM(S): Mus musculus 
2011-01-10 | E-GEOD-25310 | biostudies-arrayexpress
TranscriptomicsTranscriptome-wide discovery of microRNA binding sites in human brain by Ago2 HITS-CLIP [Ago2-miRNA complexes]
Here, seeking to gain insight into the array of transcripts engaged with miRNAs in human brain, we performed HITS-CLIP to profile transcriptome-wide Ago2:RNA interactions in a panel of eleven post-mortem adult human brain samples harvested from adult motor cortex and cingulate gyrus, regions associa...
ORGANISM(S): Homo sapiens 
2014-01-03 | E-GEOD-52083 | biostudies-arrayexpress
TranscriptomicsAgo2 associated gene transcripts in two Hodgkin lymphoma cell lines
The aim of the study is to identify miRNA targets in Hodgkin lymphoma cell lines. By immunoprecipitation of wild type Ago2, it is expected to pull down the Ago2 associated gene transcripts. Through microarray analysis, the Ago2 associated gene transcripts identified are expected to be miRNA targets....
ORGANISM(S): Homo sapiens 
2010-04-08 | E-GEOD-14409 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected
As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resi...
ORGANISM(S): Homo sapiens 
2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress
TranscriptomicsIdentification of miRNA targets in breast cancer cells (Ago2-IP)
miRNAs regulate mRNA stability and translation through the action of the RNAi-induced silencing complex. In this study, we systematically identified endogenous miRNA target genes by using AGO2 immunoprecipitation (AGO2-IP) and microarray analyses in two breast cancer cell lines, MCF7 and MDA-MB-231,...
ORGANISM(S): Homo sapiens 
2013-10-21 | E-GEOD-48158 | biostudies-arrayexpress
Sort   by:  
 Page size