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ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050933 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
ProteomicsQuantitative comparative proteomic analysis of ITGB1-knockdown and ITGB1-vector control cells in K562/S
The global proteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. The effect of ITGB1 on imatinib resistance to leukemia was checked by comparing quantitative proteomic analysis of ITGB1-knockdown (ITGB1-KD) and ITGB1- vector c...
ORGANISM(S): Homo sapiens (Human) 
2025-10-30 | PXD057116 | Pride
Swath Lc-ms/ms. Proteomics 
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Cercopithecus aethiops (Green monkey) (Grivet) Pinus radiata (Monterey pine) Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Dictyostelium discoideum (Slime mold) Cicer arietinum (Chickpea) (Garbanzo) Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Brassica oleracea var. botrytis (Cauliflower) Bombyx mori (Silk moth) Brassica napus (Rape) 
2025-10-30 | PXD057032 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
ProteomicsImmunoprecipitation followed by mass spectrometry (IP-MS)
Immunoprecipitation of ITGB1 and YWHAE was done to understand its interactive partners in K562/S cells. Mass spectrometry was performed to identify interactive molecules. Non-specific proteins (contaminants) such as keratins were excluded from the final analysis based on the results of the negative ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-30 | PXD056828 | Pride
Immunoprecipitation Mass spectrometry Protein-protein interaction Lc-ms/ms 
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