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Many epithelia have contact with air-liquid interfaces. This applies particularly to the lung, where one of the epithelial cell types (the surfactant secreting alveolar type II [AT II] cells) even project into the air. This specific environment may be of considerable physiological relevance; however...
ORGANISM(S): Rattus norvegicus 
Effect of air-liquid interface stimulation on gene expression during keratinocyte differentiation by three-dimensional culture
To investigate how air-liquid interface stimulation induces epidermal differentiation, we carried out RNA-seq on three-dimensional culture with and without air exposure.
ORGANISM(S): Homo sapiens 
2022-12-08 | GSE205727 | GEO
Transcriptomic Analysis of Air–Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation
Microarray was used to study global gene expression of a cell culture model based on SV40-immortalized human corneal epithelial (iHCE) cells. The gene expression profile of the cell line was compared to the normal human corneal epithelium. Affymetrix HG-U133A GeneChips® were used for microarray exp...
ORGANISM(S): Homo sapiens 
Raw RNAseq data files of genetically unsolved PCD patients used for transcriptomic analysis aming to uplift the diagnostic rate. The non-PCD patients were used as a clinical comparator to the PCD patients. Majority of the nasal epithelial cells used for RNAseq were cultured at an air-liquid interfac...
Although desert dust is known to cause increased respiratory morbidity and mortality, the underlying biological pathways remain unclear. We used RNA-seq on an advanced human alveolar in vitro model to find yet unidentified genes dysregulated by Saharan dust exposure. For comparison, DQ12 quartz dust...
ORGANISM(S): Homo sapiens 
Single-cell RNA-seq of air-liquid interface bronchioalveolar cells
We comprehensively analyzed differentiation effects of the air–liquid interface (ALI) culture method on adult tissue-derived human lung organoids and examined the similarity with in vivo human airway epithelium compared to submerged cultures using single-cell RNA sequencing.
ORGANISM(S): Homo sapiens 
2024-12-02 | GSE280502 | GEO
scRNA-seq of canalicular stage human fetal lung cultured under air-liquid interface
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