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TranscriptomicsChromatin immunoprecipitation of the MSL-1 and MSL-3 vs a mock IP in Drosophila and transcription profiling of X linked genes in WT male or female III instar larvae to provide X chromosome wide profiling of MSL-1 distribution and dosage compensation in Drosophila
Dosage compensation ensures that males and females, despite unequal number of X chromosomes, equalize for X linked gene expression. In Drosophila, it is achieved by a two-fold up-regulation of most of the genes present on the male X chromosome, and requires the association of the Dosage Compensation...
ORGANISM(S): Drosophila melanogaster 
2006-04-01 | E-MEXP-554 | biostudies-arrayexpress
ProteomicsCAPRI RNA binding proteins and domains in Drosophila and Humans
In the current work, we present a comprehensive coverage of the RNA-binding protein (RBP) interactome of Drosophila at four levels of resolution. The highest level tackled in our study represents the RNA-protein complexes, which we address by combining formaldehyde crosslinking of Drosophila S2 cell...
ORGANISM(S): Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2019-07-02 | PXD013338 | Pride
Rna crosslinked peptides Rna protein interaction 
TranscriptomicsChromatin immunoprecipitation to determine MSL1, MSL3, MOF and H4K16Ac binding in Drosophila SL2 and Kc cells reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila
Genome-wide binding profiles of MSL1, MSL3, MOF and H4K16Ac in Drosophila SL2 and Kc cells.
ORGANISM(S): Drosophila melanogaster 
2008-06-12 | E-MEXP-1508 | biostudies-arrayexpress
TranscriptomicsTiling path and RNAi of Drosophila KO MSL1, MLS3, and MOF reveals MOF as a key regulator of dosage compensation and gene expression
Effect of MSL1, MSL3 and MOF RNAi KOs in Drosophila SL-2 and Kc cells.
ORGANISM(S): Drosophila melanogaster 
2008-06-09 | E-MEXP-1505 | biostudies-arrayexpress
GenomicsChIP-seq profiling of RNA Polymerase II in Drosophila melanogaster S2 cells depleted of the non-specific lethal (NSL) complex
We show that the non-specific lethal (NSL) complex in Drosophila binds to housekeeping genes. Individual ChIP-qPCR experiments of NSL target genes indicated a role of the NSL complex in RNA Polymerase II (Pol II) recruitment to promoters. Consequently, we obtained ChIP-Seq profiles of the Pol-II-sub...
ORGANISM(S): Drosophila melanogaster 
2012-06-19 | E-MTAB-1084 | biostudies-arrayexpress
GenomicsChIP-Seq of NSL3 and MBD-R2 in Drosophila melanogaster S2 cells to investigate the function of Non-Specific Lethal (NSL) complex
The major H4K16 acetylase MOF has been found in two complexes, the Male-Specific Lethal (MSL) and the Non-Specific Lethal (NSL) complex. The latter one consists of at least 7 members: NSL1, NSL2, NSL3, MBD-R2, MCRS2, WDS, and MOF. To investigate it's function, we performed genome-wide profiling of N...
ORGANISM(S): Drosophila melanogaster 
2012-06-19 | E-MTAB-1085 | biostudies-arrayexpress
TranscriptomicsMapping of MLE and MSL2 using iCLIP
Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long non-coding RNAs (lncRNAs) for transcriptional up-regulation of the entire X-chromosome. Here, UV cross-linking followed by deep sequencing (iCLIP) show that two enzymes in the Male-Specific Lethal complex, MLE ...
ORGANISM(S): Drosophila melanogaster 
2013-07-18 | E-MTAB-1669 | biostudies-arrayexpress
GenomicsMLE ChIP-seq
Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) was carried out on wild-type Schneider (S2) cells using specific MLE antibodies to identify binding sites for MLE in the Drosophila genome
ORGANISM(S): Drosophila melanogaster 
2013-10-30 | E-MTAB-1705 | biostudies-arrayexpress
TranscriptomicsMLE HITS-CLIP in Drosophila S2 cells
HITS-CLIP was carried out on wild-type Schneider (S2) cells using specific MLE antibodies to identify binding sites for MLE in the Drosophila transcriptome
ORGANISM(S): Drosophila melanogaster 
2013-10-30 | E-MTAB-1706 | biostudies-arrayexpress
TranscriptomicsAnalysis of MOF under stress conditions
We analyzed the role of MOF in primary MEFs and differentiated podocytes in response to Adriamycin. Mof was deleted in MEFs using the Cre-ERT2 trasgene, while Mof was knockdown in podocytes using shRNA infection. Samples were treated with Adriamycin for 24 hours and gene expression changes analysed....
ORGANISM(S): Mus musculus 
2015-07-10 | E-GEOD-70706 | biostudies-arrayexpress
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