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ProteomicsAnalysis of fluorophore-labeled human cytochrome b5
Cytochrome b5 (b5) is a small accessory protein involved in several reactions. The interaction of b5 with its partner proteins, e.g., cytochrome P450 enzymes, has received much attention and has been the subject of numerous studies. In order to obtain further insights into protein-protein interactio...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD047758 | Pride
Cytochrome b5 Fluorophore Human Alexa 488 
TranscriptomicsSequence specificity incompletely defines the genome-wide occupancy of Myc
The Myc-Max heterodimer is a DNA binding protein that regulates expression of a large number of genes. Genome occupancy of Myc-Max is thought to be driven by E-boxes (CACGTG or variants) to which the heterodimer binds in vitro. By analyzing ChIP-Seq datasets, we demonstrated that the positions occup...
ORGANISM(S): Homo sapiens 
2014-09-22 | E-GEOD-58570 | biostudies-arrayexpress
UnknownAlexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Not available
S-EPMC9083145 | biostudies-literature
TranscriptomicsSprouting transcriptome in cortical neurons: young
Selective isolation of total RNA and then whole genome expression analysis of sprouting neurons in peri-infarct cortex of the adult rat after stroke, compared to adjacent neurons that have not sprouted a new connection, in young adult (2 months) and aged (2 years) animals. Two different fluorescent ...
ORGANISM(S): Rattus norvegicus 
2010-11-15 | E-GEOD-24440 | biostudies-arrayexpress
TranscriptomicsSprouting transcriptome in cortical neurons: aged
Selective isolation of total RNA and then whole genome expression analysis of sprouting neurons in peri-infarct cortex of the adult rat after stroke, compared to adjacent neurons that have not sprouted a new connection, in young adult (2 months) and aged (2 years) animals. Two different fluorescent ...
ORGANISM(S): Rattus norvegicus 
2010-11-15 | E-GEOD-24441 | biostudies-arrayexpress
UnknownQuantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores.
Not available
S-EPMC4403856 | biostudies-literature
TranscriptomicsMicroarray gene expression profiling of 27k cDNAs in 23 healthy porcine tissues
Background. Gene expression microarrays have been intensively applied to screen for genes involved in specific biological processes of interest such as diseases or responses to environmental stimuli. For mammalian species, cataloging of the global gene expression profiles in large tissue collections...
ORGANISM(S): Sus scrofa 
2007-11-21 | E-GEOD-4918 | biostudies-arrayexpress
TranscriptomicsRNA-seq of acLDL high vs low 4T1-T cells
To determine whether acLDL uptake was associated with specfifc patterns of gene expression we treated vasculogenic mimicry-competent 4T1-T mammary carcinoma cells with Alexa-488 labelled acetylated low density lipoproteins (acLDL) and sorted the cells based on high or low uptake and subjected them t...
ORGANISM(S): Mus musculus 
2023-07-26 | GSE232213 | GEO
TranscriptomicsProteinM-bM-^HM-^RDNA binding in the absence of specific base-pair recognition
Until now, it has been reasonably assumed that specific base-pair recognition is the only mechanism controlling the specificity of transcription factor (TF)M-bM-^HM-^RDNA binding. Contrary to this assumption, here we show that nonspecific DNA sequences possessing certain repeat symmetries, when pres...
ORGANISM(S): Homo sapiens 
2014-10-01 | E-GEOD-61920 | biostudies-arrayexpress
TranscriptomicsStability selection for regression-based models of transcription factor-DNA binding specificity
Motivation: The DNA binding specificity of a transcription factor (TF) is typically represented using a position weight matrix (PWM) model, which implicitly assumes that individual bases in a TF binding site contribute independently to the binding affinity, an assumption that does not always hold. F...
ORGANISM(S): Homo sapiens 
2013-05-17 | E-GEOD-47026 | biostudies-arrayexpress
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