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GenomicsIn vivo ChIP-seq analysis of Foxa1 and Foxa2 binding sites in midbrain dopaminergic progenitors and neurons
E12.5 wild-type embryos were dissected to collect ventral midbrain regions. Samples were crosslinked 10min with 1% formaldehyde and processed for chromatin extraction and Chromatin Immunoprecipitation (ChIP) following the Millipore upstate protocol. Libraries were prepared using the illumina ChIP-Se...
ORGANISM(S): Mus musculus 
2015-03-02 | E-MTAB-3332 | biostudies-arrayexpress
TranscriptomicsIn vivo RNA-Seq analysis of Foxa1 and Foxa2 transcriptional target at E12.5
E12.5 wild-type and NestinCre Foxa1/2 Flox/ Flox mutant embryos were quickly snap-frozen, then 10um-thick cryostat coronal sections of the midbrain were cut and mounted on membrane slides (Zeiss) and the floor plate was Laser Capture Microdissected. RNA was extracted using the Picopure RNA Isolation...
ORGANISM(S): Mus musculus 
2015-02-19 | E-MTAB-3333 | biostudies-arrayexpress
GenomicsChIP-seq analysis of Foxa2 binding sites in midbrain dopaminergic progenitors
Embryonic stem (ES) cells were differentiated in culture to midbrain dopaminergic (mDA) progenitors and subjected to ChIP-seq analysis to resolve genome-wide binding sites of forkhead box protein A2 (Foxa2). Foxa2 was found to directly regulate multiple lineage pathways to specify midbrain dopaminer...
ORGANISM(S): Mus musculus 
2012-07-01 | E-MTAB-1137 | biostudies-arrayexpress
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