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ProteomicsA Novel Approach for Generating Antigen Interference-Free Anti-Peptide Polyclonal Antibodies for Quantitative Measurements of Low-Abundance Protein Biomarkers Using Ultra-Sensitive Anti-Peptide Immunocapture LC-MS/MS Assays
Antigen interference from residual antigen peptides remains a persistent challenge in anti-peptide immunocapture liquid chromatography-tandem mass spectrometry (IC-LC-MS/MS) assays for quantifying low-abundance protein biomarkers. In this manuscript, we introduce a novel approach that utilizes carri...
ORGANISM(S): Homo sapiens (Human) 
2026-09-04 | PXD076272 | Pride
Stable isotopically labeled peptide antigen Anti-peptide immunocapture Protein biomarker Antigen interference Lc-ms/ms 
UnknownShaping Polyclonal Responses via Antigen-Mediated Antibody Interference
Not available
S-EPMC7530306 | biostudies-literature
TranscriptomicsProfiling of circadian genes expressed in the uterus endometrial stromal cells of pregnant rats as revealed by DNA microarray coupled with RNA interference
The peripheral circadian oscillator plays an essential role in synchronizing local physiology to operate in a circadian manner via regulation of the expression of clock-controlled genes. In the murine uterus, the endometrial stromal cells undergo proliferation and differentiation into decidual cells...
ORGANISM(S): Rattus norvegicus 
2013-07-23 | E-GEOD-49080 | biostudies-arrayexpress
TranscriptomicsInterference of B-Cell and T-Cell Receptor Genes in Single-Cell RNA Sequencing Data
B and T cells play central roles in immune responses and, as such, are integral in many diseases, such as autoimmunity. Characterizing these cells, their subsets, and exploring their transcriptomes to understand the underlying biology, can be achieved using single-cell RNA sequencing (scRNA-seq). Wh...
ORGANISM(S): Homo sapiens 
2025-12-16 | E-MTAB-12346 | biostudies-arrayexpress
TranscriptomicsDEPS-1 promotes P granule assembly and RNA interference in C. elegans germ cells
P granules are germ-cell-specific cytoplasmic structures containing RNA and protein and required for proper germ cell development in C. elegans. A new P-granule-associated protein, DEPS-1, whose loss disrupts P granule structure and function was analysed in this study. Genome wide analysis of gene...
ORGANISM(S): Caenorhabditis elegans 
2010-05-26 | E-GEOD-9993 | biostudies-arrayexpress
Transcriptomicssi RNA interference of Hepatitis B viral gene expression
Gene expression profiling comparisons of HepG2.2.15 or PLC/PRF/5 cells either mock (M) transfected or transfected with 0.2 microM S2 RNA or Scrambled (SCR) siRNA were carried out in duplicate 48 hours after transfection. The experiments were carried out in duplicate (a and b). The following combinat...
ORGANISM(S): Homo sapiens 
2006-01-06 | E-GEOD-3992 | biostudies-arrayexpress
UnknownInhibition of lipid antigen presentation in dendritic cells by HIV-1 Vpu interference with CD1d recycling from endosomal compartments.
Not available
S-EPMC3173986 | biostudies-literature
TranscriptomicsA rapid and scalable system for studying gene function in mice using conditional RNA interference
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luc...
ORGANISM(S): Mus musculus 
2010-12-01 | E-GEOD-19476 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human mesenchymal stem cell-derived osteoblasts following RNAi knock down of phosphodiesterase 7 and 8A
Effects of phosphodiesterase 7 and 8A inhibition by RNA Interference on the gene expression of human mesenchymal stem cell-derived osteoblasts.
ORGANISM(S): Homo sapiens 
2008-12-12 | E-MEXP-1021 | biostudies-arrayexpress
TranscriptomicsHeLa cell mitochondrial microRNAs
MicroRNAs (miRNAs) are small non-coding RNAs that associate with Argonaute 2 protein to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in other cellular compartments. Mitochondria harbour th...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-24761 | biostudies-arrayexpress
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