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Here we sought to identify mesophyll messenger(s) that promote stomatal opening under red light. We first established a method to isolate leaf apoplastic fluids from Arabidopsis thaliana and Vicia faba and ascertained that the apoplastic fluid enhances red light-induced stomatal opening. We then app...
2025-06-23 | MTBLS5383 | MetaboLights
Soybean leaves were inoculated with P. sojae strain P6497, and apoplastic fluid was collected at 1.5, 3, and 6 hours post-inoculation (hpi). The samples were analyzed using MS to identify candidate apoplastic effectors and PsTry1.
ORGANISM(S): Phytophthora sojae strain P6497 
2025-06-14 | PXD058375 | Pride
Papain-like cysteine proteases (PLCPs) play important roles in plant defense mechanisms. Previous work identified a set of five apoplastic PLCPs (CP1A, CP1B, CP2, XCP2 and CatB) which are crucial for the orchestration of SA-dependent defense signaling and vice versa in maize (Zea mays). One central ...
ORGANISM(S): Zea mays (Maize) 
2019-02-15 | PXD006751 | Pride
Many genes involve in pathogenicity and virulence are induced only in plant or in the presence of host components. Plant apoplast is the primary site of infection for P. syringae, which obtain nutrients directly from apoplastic fluid of host plants. In this work we investigated the effect of apoplas...
ORGANISM(S): Pseudomonas savastanoi pv. phaseolicola 
2009-03-03 | GSE14983 | GEO
N-glycosylation site identification in Arabidopsis thaliana apoplastic fluid proteome by legumain using two alternative workflows to cross validate with dimethylation.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-20 | PXD014680 | Pride
Although it is known that plants can recruit beneficial rhizobacteria under various stresses, the mechanisms underlying their synergistic interaction in regulating plant resistance remain poorly understood. This study demonstrates that plants establish metabolic crosstalk with Pseudomonas for enhanc...
2026-09-12 | MTBLS15622 | MetaboLights
Wheat leaf rust caused by the pathogenic fungus Puccinia triticina, is a serious threat to bread wheat and durum production in many areas of the world. This plant-pathogen interaction has been studied extensively at the molecular genetics level, however proteomics data are still relatively scarce. ...
ORGANISM(S): Puccinia triticina Triticum aestivum (Wheat) 
2019-09-13 | PXD012586 | Pride
In order to identify the key factors that are crucial for non-host resistance against Bgh and are secreted depending on SYP4-mediated membrane trafficking, we have performed apoplastic fluid preparation of a series of mutants defective in the SYP4 pathway. This project aims to identify proteomic pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-11 | PXD009099 | Pride
Arabidopsis Apoplastic Fluid Contains sRNA- and Circular RNA-Protein Complexes that Are Located Outside Extracellular Vesicles
The extracellular space within plant leaves is called the apoplast, and functions as a key battlefield between plants and pathogens. Previously, we have shown that apoplastic wash fluid purified from Arabidopsis leaves contains small RNAs (sRNAs). To investigate whether these RNAs are encapsulated i...
ORGANISM(S): Arabidopsis thaliana 
2022-04-18 | GSE185133 | GEO
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