Sort   by:  
 Page size 
TranscriptomicsProduction of artificial piRNAs in flies and mice
In animals, a discrete class of small RNAs, the piwi-interacting RNAs (piRNAs), guard germ cell genomes against the activity of mobile genetic elements. piRNAs are generated, via an unknown mechanism, from apparently single-stranded precursors that arise from discrete genomic loci, termed piRNA clus...
ORGANISM(S): Mus musculus 
2012-01-01 | E-GEOD-32435 | biostudies-arrayexpress
TranscriptomicsA piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice
We examined the construction of the piRNA system in the restricted developmental window in which methylation patterns are set during mammalian embryogenesis. We find robust expression of two Piwi family proteins, MIWI2 and MILI. Their associated piRNA profiles reveal differences from Drosophila wher...
ORGANISM(S): Mus musculus 
2008-10-01 | E-GEOD-12757 | biostudies-arrayexpress
TranscriptomicspiRNA libraries from Drosophila melanogaster ovaries
Drosophila Piwi-family proteins have been implicated in transposon control. Here, we examine piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and find that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predomin...
ORGANISM(S): Drosophila melanogaster 
2007-03-08 | E-GEOD-6734 | biostudies-arrayexpress
TranscriptomicsPiwi induces piRNA-guided transcriptional silencing and establishment of a repressive chromatin state
In the Metazoan germline, piwi proteins and associated piwi-interacting RNAs (piRNAs) provide a defense system against the expression of transposable elements. In the cytoplasm, piRNA sequences guide piwi complexes to destroy complementary transposon transcripts by endonucleolytic cleavage. However,...
ORGANISM(S): Drosophila melanogaster 
2013-02-11 | E-GEOD-43829 | biostudies-arrayexpress
TranscriptomicsProteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members
In germ cells, Piwi proteins interact with a specific class of small non-coding RNAs, piwi-interacting RNAs (piRNAs). Together, these form a pathway that represses transposable elements, thus safeguarding germ cell genomes. While basic models describe the operation of piRNA pathways, neither the pr...
ORGANISM(S): Mus musculus 
2009-07-25 | E-GEOD-17319 | biostudies-arrayexpress
TranscriptomicsSmall RNA-based silencing strategies for transposons in the process of invading Drosophila species
We investigated a pool of small RNAs derived from transposon Penelope to probe the evolution of small RNA pathways in response to the transposon challenge in natural (D. virilis) and experimentally induced (D. melanogaster) colonization processes. In both species, Penelope was predominantly targeted...
ORGANISM(S): Drosophila melanogaster 
2010-07-18 | E-GEOD-22067 | biostudies-arrayexpress
Sort   by:  
 Page size