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TranscriptomicsThe epigenetic signature of subcutaneous fat cells is linked to altered expression of genes implicated in lipid metabolism in obese women
Background Obesity is associated with changes in fat cell gene expression and metabolism. What drives these changes is not well understood. We aimed to explore fat cell epigenetics, i.e., DNA methylation, as one mediator of gene regulation, in obese women. The global DNA methylome for abdominal subc...
ORGANISM(S): Homo sapiens 
2015-09-15 | E-GEOD-67024 | biostudies-arrayexpress
TranscriptomicsThe fat cell epigenetic signature in post-obese women is characterized by global hypomethylation and differential DNA methylation of adipogenesis genes
Background/Objectives: Obese subjects have increased number of enlarged fat cells which are reduced in size but not number in post-obesity. We performed DNA methylation profiling in fat cells with the aim of identifying differentially methylated DNA sites (DMS) linked to adipose hyperplasia (many sm...
ORGANISM(S): Homo sapiens 
2015-05-06 | E-GEOD-58622 | biostudies-arrayexpress
TranscriptomicsAdipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity (differentiation data)
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Subcutaneous adipose tissue was obtained from healthy subjects undergoing co...
ORGANISM(S): Homo sapiens 
2012-08-12 | E-GEOD-25910 | biostudies-arrayexpress
TranscriptomicsAdipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression]
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Abdominal subcutaneous adipose needle biopsies were obtained from women (n=5...
ORGANISM(S): Homo sapiens 
2012-08-12 | E-GEOD-25401 | biostudies-arrayexpress
TranscriptomicsEarly B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [expression profiling]
To investgate the role of EBF1 in human adipocyte, we performed global expression profiling in human adipocytes transfected with siRNA targeting EBF1. Human adipocytes differentiated in vitro were transfected with siRNA targeting EBF1 or Non-targeting Control. The cells were incubated for approximat...
ORGANISM(S): Homo sapiens 
2014-05-22 | E-GEOD-42680 | biostudies-arrayexpress
GenomicsEarly B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [ChIP-seq]
Examination of EBf1 binding by ChIP-seq in differentiated human adipose stromal cell (hASC) pre-adipocyte Pre-Adipocytes differentiated in-vitro were fixed in 1% formaldehyde for 15 min at room temperature and quenched for 5 min by adding glycine to a final concentration of 0.125 M. ChIP assays wer...
ORGANISM(S): Homo sapiens 
2014-05-22 | E-GEOD-54889 | biostudies-arrayexpress
TranscriptomicsThe epigenetic signature of systemic insulin resistance in obese women [BL]
Impaired ability of insulin to stimulate cellular glucose uptake and regulate metabolism, that is insulin resistance (IR), links adiposity to metabolic disorders such as type 2 diabetes (T2D), dyslipidemia and cardiovascular disease (Langenberg, 2012). Both genetic and epigenetic factors are implica...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-76285 | biostudies-arrayexpress
TranscriptomicsThe epigenetic signature of systemic insulin resistance in obese women [SC]
Impaired ability of insulin to stimulate cellular glucose uptake and regulate metabolism, that is insulin resistance (IR), links adiposity to metabolic disorders such as type 2 diabetes (T2D), dyslipidemia and cardiovascular disease (Langenberg, 2012). Both genetic and epigenetic factors are implica...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-76398 | biostudies-arrayexpress
TranscriptomicsThe epigenetic signature of systemic insulin resistance in obese women [OM]
Impaired ability of insulin to stimulate cellular glucose uptake and regulate metabolism, that is insulin resistance (IR), links adiposity to metabolic disorders such as type 2 diabetes (T2D), dyslipidemia and cardiovascular disease (Langenberg, 2012). Both genetic and epigenetic factors are implica...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-76394 | biostudies-arrayexpress
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