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TranscriptomicsTranscription profiling of Actinobacillus pleuropneumoniae wild type, hns mutants and rseA mutants
Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-N-acetylglucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form a bi...
ORGANISM(S): Actinobacillus pleuropneumoniae 
2010-04-22 | E-MEXP-2461 | biostudies-arrayexpress
TranscriptomicsExpression signatures of human HeLa cells with RNAi targetting A1 hnRNP, FUS, H hnRNP and p68-DDX5
Transcription profiling of human HeLa cells (cervical cancer cell line) transfected with a plasmid expressing shRNAs cloned into the pSuper expression vector compared to emprty vector negative controls for transfection. Four different RNA interference treatments targetted: A1 hnRNP (HNRNPA1, Heterog...
ORGANISM(S): Homo sapiens 
2008-08-20 | E-GEOD-12058 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Wheat-Phytase transgenic plants vs a non-transgenic control line at three different developmental stages of the seed
A transgenic wheat line was compared for gene expression patterns to a non-transgenic control line at three different developmental stages of the seed. The three successive developmental stages were also compared for each of the two lines. Thus, a factorial experiment with three developmental stages...
ORGANISM(S): Triticum aestivum 
2005-07-01 | E-MEXP-368 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli strain MC4100 in comparison to its otherwise isogenic ycgE::cat derivative to identify YcgE dependent genes
Genome-wide transcriptional profiling on microarray was performed for strain MC4100 in comparison to its otherwise isogenic ycgE::cat derivative. Strains were grown in LB at 28°C and cells were harvested at an OD (578nm) of 4.0.
ORGANISM(S): Escherichia coli 
2009-06-03 | E-MEXP-1872 | biostudies-arrayexpress
TranscriptomicsTranscriptional responses of Mycobacterium tuberculosis to lung surfactant
This study uses microarray analyses to examine gene expression profiles for Mycobacterium tuberculosis (Mtb) induced by exposure in vitro to bovine lung surfactant preparations that vary in apoprotein content: (i) whole lung surfactant (WLS) containing the complete mix of endogenous lipids and surfa...
ORGANISM(S): Mycobacterium tuberculosis 
2010-05-18 | E-GEOD-14005 | biostudies-arrayexpress
TranscriptomicsAnaerobic transcriptional responses of Escherichia coli to NO under defined chemostat conditions.
Escherichia coli strain MG1655 was grown in a New Brunswick Scientific Bioflow III Biofermentor under continuous culture (chemostat) conditions. Cells were grown in defined media containing 54 mM glycerol as the sole and limiting source of energy and carbon. The working volume was 1 litre, and the d...
ORGANISM(S): Escherichia coli 
2010-07-01 | E-GEOD-5076 | biostudies-arrayexpress
MetabolomicsWhole blood metabolomics of dementia patients reveal classes of disease-linked metabolites

Dementia is caused by factors that damage neurons. We quantified small molecular markers in whole blood of dementia patients, using non-targeted liquid chromatography-mass spectroscopy (LC-MS). Thirty-three metabolites, classified into 5 groups (A-E), differed significantly in dementia patients, ...

2021-07-06 | MTBLS2109 | MetaboLights
TranscriptomicsTransformation of Naked Mole-Rat Cells
The naked mole-rat (NMR), Heterocephalus glaber, is a mouse-sized subterranean rodent native to East Africa. Research on NMRs is intensifying in an effort to gain leverage from their unusual physiology, long-life span and cancer resistance for the development of new theraputics. Few studies have att...
ORGANISM(S): Mus musculus 
2020-06-12 | E-MTAB-8932 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of human leukemic B-cells.
Fresh blood samples were collected from 10 CLL (chronic lymphocytic leukemia) previously untreated patients before and after 2 weeks of the first cycle of CCR (cladribine, cyclophosphamide, rituximab) treatment. Peripheral blood mononuclear cells (PBMNCs) were separated from EDTA blood by layering o...
ORGANISM(S): Homo sapiens 
2013-12-31 | E-GEOD-33925 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Staphylococcus aureus in response to unsaturaed long chain free fatty acids
Data is also available from http://bugs.sgul.ac.uk/E-BUGS-68
ORGANISM(S): Staphylococcus aureus 
2009-02-04 | E-BUGS-68 | biostudies-arrayexpress
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