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ProteomicsAtta columbica seminal fluid and spermathecal fluid proteome
Mature males and virgin queens from Atta colombica ants were collected in panama, and their seminal fluid (SF) and spermathecal fluid (SpF), respectively, were extracted and analysed by shot-gun proteomics. For this, using a pooled SF sample of 25 males from five colonies, squeezing their abdomens t...
ORGANISM(S): Atta columbica 
2019-01-03 | PXD011306 | Pride
Sexual conflict Dige Spermathecal fluid. Atta colombica Seminal fluid 
ProteomicsDIGE based quantitation of Atta columbica ant male seminal fluid exposed to queen spermatheca fluid.
Polyandrous ant queens are inseminated early in their life and store sperm mixtures for a potential reproductive life of decades. However, they cannot re-mate later in life and are thus expected to control the loss of viable sperm because their life-time reproductive success is ultimately limited by...
ORGANISM(S): Atta columbica 
2019-01-03 | PXD011320 | Pride
Sexual conflict Dige Spermathecal fluid. Atta colombica Seminal fluid 
ProteomicsAnalysis of the honeybee sperm proteome using MudPit and 2D PAGE
Honeybee semen was collected by gently squeezing the male abdomens. Samples were pooled and then centrifuged. Pelleted sperm was collected and analysed with both 2D PAGE and gel-free methods. For the 2D PAGE, protein spots were digested using trypsin. Extracted peptides, resolved on a C18 column, we...
ORGANISM(S): Apis mellifera (Honeybee) 
2020-01-17 | PXD000169 | Pride
Sperm. lc-ms 2d page Mudpit Honey bee 
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