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ProteomicsProximity labeling and orthogonal nanobody pulldown (ID-oPD) approaches to map the spinophilin interactome uncover a role for spinophilin in protein regulation.
The major synaptic protein phosphatase 1 (PP1) targeting protein, spinophilin can inhibit or target PP1 activity towards diverse substrates. To detail the full panoply of substrates at which spinophilin modulates PP1 activity, we have validated a novel approach that permits proximity labeling and or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-02 | MSV000096575 | MassIVE
ProteomicsPERK protein kinase facilitates keratinocyte collective cell migration by engagement with cell adhesion molecules independent of its kinase activity
Proximity labeling experiments (BioID) were performed on Human NTERT keratinocytes depleted of PERK (EIF2AK3, PERK-KO) re-expressing a WT PERK containing a C-terminal UltraID tag. PERK-KO and PERKUltraID cells were treated with differentiation medium containing 50 uM of exogenous Biotin for 6 h, wa...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-11 | MSV000098126 | MassIVE
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