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Seed size is important to crop domestication and natural selection and is affected by the balance of maternal and paternal genomes in endosperm. Endosperm, like placenta in mammals, provides reserves to the developing embryo. Interploidy crosses disrupt the genome balance in endosperm and alter seed...
ORGANISM(S): Arabidopsis thaliana 
DCL3 is the main processor of miRNA precursors in Chlamydomonas reinhardtii. Because of that, and in order identify mRNA targets of miRNA-guided slicer complexes, we used RNAseq of the transcriptome in dcl3 mutant and parental lines.
ORGANISM(S): Chlamydomonas reinhardtii 
We grafted Transcriptional Gene Silencing (TGS)-inducing wild type Arabidopsis and a mutant that is compromised in 24 nucleotide (nt) small RNA (sRNA) production onto a wild type reporter line. We observed that 21-24 nt sRNAs were transmitted across a graft union yet only the 24 nt sRNAs directed RN...
ORGANISM(S): Arabidopsis thaliana 
A silencing signal in plants with an RNA specificity determinant moves through plasmodesmata and the phloem. To identify the mobile RNA we grafted Arabidopsis thaliana shoots to roots that would be a recipient for the silencing signal. Using high throughput sequencing as a sensitive detection method...
ORGANISM(S): Arabidopsis thaliana 
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