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ProteomicsProteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells
Here, we develop a new in vitro model replicating the first interaction of FhNEJ with mouse primary small intestinal epithelial cells (MPSIEC). FhNEJ and MPSIEC were incubated for 3 hours and protein extracts (tegument and soma for FhNEJ and membrane and cytosol for MPSIEC) were subjected to quantit...
ORGANISM(S): Fasciola hepatica (Liver fluke) 
2022-09-20 | PXD033952 | Pride
Fasciola hepatica  newly excysted juveniles  swath-ms  quantitative proteomics  differential expression  host-parasite interaction 
ProteomicsStudy of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model
The project presents the set-up of an ex vivo model coupled with quantitative SWATH-MS proteomics to study the early events following the passage of Fasciola hepatica Newly Excysted Juveniles (NEJ) though the host’s intestinal wall, assessing the proteomic changes in their surface (tegument) and som...
ORGANISM(S): Fasciola hepatica (Liver fluke) 
2022-08-31 | PXD033945 | Pride
Fasciola hepatica  newly excysted juveniles  gut passage  swath-ms  quantitative proteomics  differential expression 
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