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GenomicsIdentification of MEIS2 binding sites through ChIP-seq in the neuroblastoma Kelly and BE(2)-C cell lines.
In total, 100x106 SK-N-BE(2)-C and Kelly neuroblastoma cells were processed for ChIP-sequencing. Briefly, pellets were prepared by fixing cells in 1% formaldehyde for 15 minutes, and quenching in 1M Tris pH 7.5. Nuclei were prepared using the Sigma Nuclei Isolation Kit (#NUC-101), and sonicated usin...
ORGANISM(S): Homo sapiens 
2022-04-14 | E-MTAB-10620 | biostudies-arrayexpress
Transcriptomics4C sequencing for the SOX11 promoter in the neuroblastoma cell lines CLB-GA, KELLY, SK-N-AS and SH-EP
4C sequencing for the SOX11 promoter in the neuroblastoma cell lines CLB-GA, KELLY, SK-N-AS and SH-EP.
ORGANISM(S): Homo sapiens 
2023-02-19 | E-MTAB-12580 | biostudies-arrayexpress
TranscriptomicsIdentification of CTCF binding sites through CUT&RUN sequencing in the neuroblastoma cell line CLB-GA.
CUT&RUN sequencing to define binding sites for CTCF and IgG in the neuroblastoma cell line CLB-GA.
ORGANISM(S): Homo sapiens 
2023-02-23 | E-MTAB-12586 | biostudies-arrayexpress
TranscriptomicsIdentification of SOX11 binding sites through CUT&RUN sequencing in the neuroblastoma cell lines IMR-32, CLB-GA, NGP and SH-EP
CUT&RUN sequencing to define binding sites for SOX11 and IgG in the neuroblastoma cell lines IMR-32, CLB-GA and NGP as well as neuroblastoma cell line SH-EP after SOX11 overexpression for 48h. A SOX11 inducible overexpression system was generated using a Tet-on system.
ORGANISM(S): Homo sapiens 
2022-12-13 | E-MTAB-11905 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human CLB-GA and NGP neuroblastoma cell lines with SOX11 knockdown as compared to control
RNA-seq upon SOX11 knockdown in the neuroblastoma cell lines CLB-GA and NGP. Cells were nucleofected with a pool of four different siRNAs targeting SOX11 and a non-targeting control (NTC). Analysis was performed 2 days upon SOX11 knockdown, including four biological replicates per condition.
ORGANISM(S): Homo sapiens 
2022-12-13 | E-MTAB-11883 | biostudies-arrayexpress
TranscriptomicsATAC-seq in IMR-32, CLB-GA and SH-EP neuroblastoma cell lines.
ATAC-seq to define open and closed chromatin in the human IMR-32, CLB-GA and SH-EP neuroblastoma cell lines in an untreated way.
ORGANISM(S): Homo sapiens 
2023-02-23 | E-MTAB-12588 | biostudies-arrayexpress
GenomicsSOX11 ChIP-seq in neuroblastoma cell line IMR-32
ChIP-seq to define bindings sites for SOX11 and Input in the neuroblastoma cell line IMR-32
ORGANISM(S): Homo sapiens 
2021-11-30 | E-MTAB-9464 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human SH-EP neuroblastoma cell line with SOX11 overexpression as compared to control at 9h timepoint
RNA-seq to define downstream targets of adrenergic SOX11 upon 1ug/ml doxycyline inducible SOX11 overexpression in a SOX11 non-expressing mesenchymal cell line SH-EP. Analysis was performed 48h upon treatment with doxycycline, control samples are the untreated cells. 2 biological replicates were used...
ORGANISM(S): Homo sapiens 
2022-12-13 | E-MTAB-11892 | biostudies-arrayexpress
TranscriptomicsATAC-seq of human SH-EP neuroblastoma cell line with SOX11 overexpression as compared to control
ATAC-seq to define open and closed chromatin in the human SH-EP neuroblastoma cell upon 1ug/ml doxycyline inducible SOX11 overexpression. Analysis was performed 48h upon treatment with doxycycline, control samples are the untreated cells. 3 biological replicates from different monoclonal expansions ...
ORGANISM(S): Homo sapiens 
2023-12-30 | E-MTAB-11898 | biostudies-arrayexpress
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