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TranscriptomicsDrosophila pseudoobscura and Drosophila simulans small RNAs
High-throughput sequencing of Drosophila pseudoobscura and Drosophila simulans small RNAs. ~18-26nt RNAs were isolated from total RNA using PAGE, ligation to adapters requires 5' monophosphate and 3' OH. Small RNAs were cloned from Drosophila pseudoobscura (heads and pooled 0-12 and 12-24 hour embry...
ORGANISM(S): Drosophila simulans 
2009-11-24 | E-GEOD-13677 | biostudies-arrayexpress
TranscriptomicsRepertoire and evolution of miRNA genes in four divergent nematode species
We have performed small RNA sequencing in the nematodes Caenorhabditis elegans, C. briggsae, C. remanei and Pristionchus pacificus, which have diverged up to 400 million years ago, to establish the repertoire and evolutionary dynamics of miRNAs in these species. In addition to previously known miRN...
ORGANISM(S): Caenorhabditis elegans 
2009-09-07 | E-GEOD-15169 | biostudies-arrayexpress
TranscriptomicsMicroRNA-Directed siRNA Biogenesis in Caenorhabditis elegans
C.elegans small RNAs from HA::ALG-1, HA::ALG-2 and HA::RDE-1 IP and rde-1 mutants Small RNAs were cloned from transgenic or mutant C. elegans adults. Sequencing was performed using 454 and Illumina platforms.
ORGANISM(S): Caenorhabditis elegans 
2010-04-14 | E-GEOD-20649 | biostudies-arrayexpress
TranscriptomicsC. elegans small RNAs
High throughput sequencing to derive function of cde-1 in endogenous RNAi in C. elegans Small RNAs were cloned from C. elegans adults, following removal of tri-phosphate groups from 5' end. Sequencing was performed using the Illumina 1G platform.
ORGANISM(S): Caenorhabditis elegans 
2009-10-06 | E-GEOD-17787 | biostudies-arrayexpress
TranscriptomicsZebrafish germline small RNAs
Piwi proteins specify an animal-specific subclass of the Argonaute family, that in vertebrates are specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is expressed in both the male and the female gonad, and is a component of a germline specifying structure, called nuage. ...
ORGANISM(S): Danio rerio 
2007-04-04 | E-GEOD-7131 | biostudies-arrayexpress
Transcriptomicsrem-1 analysis in C. elegans
small RNA libraries from total RNA isolated from young adult animals Wild-type and rem-1 mutant animals were used for RNA isolation. Regular libraries were made using adaptor ligations at both ends. In addition, librraies were made from oxidised and TAP treated RNA.
ORGANISM(S): Caenorhabditis elegans 
2012-10-30 | E-GEOD-31783 | biostudies-arrayexpress
TranscriptomicsZebrafish Zili- and Ziwi-interacting small RNAs
Small RNAs exert an effect through diverse RNA interference pathways to transcriptionally or post-transcriptionally silence their targets. The Piwi-interacting RNAs (piRNAs) represent a germline-specific small RNA pathway where Piwi proteins themselves are thought to mediate piRNA biosynthesis. Here...
ORGANISM(S): Danio rerio 
2008-11-04 | E-GEOD-12547 | biostudies-arrayexpress
TranscriptomicsNeuronal activity regulates hippocampal miRNA expression
Small RNA fractions from 6-8 week old C57BL/6 mouse hippocampus following electroconvulsive shock (ECS) Size selected RNA clones using Illumina v1.0 DGE small RNA kit, sequenced using Illumina
ORGANISM(S): Mus musculus 
2011-10-06 | E-GEOD-32055 | biostudies-arrayexpress
TranscriptomicsHen1 analysis in zebrafish
small RNA libraries from wild-type and Hen1 mutant testes were made with either polyA tailing (VASAGFPHen1minus/plus) or adapter ligation (Hen1Testis and WTTestis) and sequenced on an Illumina GAII platform. RNA was isolated from total testis tissue of both Hen1 wildtype and Hen1 mutant animals. Aft...
ORGANISM(S): Danio rerio 
2011-11-09 | E-GEOD-33582 | biostudies-arrayexpress
TranscriptomicsTdrd1 acts as a molecular scaffold for Piwi proteins and piRNA targets in zebrafish.
RNA libraries from immunoprecipitates of Tdrd1, Ziwi and Zili, total testis RNA, total RNA from 3 week old wild-type and tdrd1 mutant gonads. Both size selected and non-size selected libraries were made. Sequencing was performed using Illumina platform.
ORGANISM(S): Danio rerio 
2011-08-31 | E-GEOD-29418 | biostudies-arrayexpress
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