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Transcriptomicsmsn2/4 mutant 3 hour exposure to zymolyase
We did transcription profiling on the effect of msn2 msn4 deletion, genes involved in several stress process Keywords: cell wall stress response S. cerevisiae (msn2/4 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600)...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-19 | E-GEOD-9934 | biostudies-arrayexpress
Transcriptomicsrlm1 mutant 3 hour exposure to zymolyase
We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-19 | E-GEOD-9933 | biostudies-arrayexpress
Transcriptomicsslt2 mutant 3 hour exposure to zymolyase
We did transcription profiling on the effect of slt2 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (slt2 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-19 | E-GEOD-9932 | biostudies-arrayexpress
Transcriptomicshog1 mutant 3 hour exposure to zymolyase
We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response S. cerevisiae (hog1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical den...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-19 | E-GEOD-9931 | biostudies-arrayexpress
Transcriptomicshog1, slt2, rlm1, and msn2/4 mutants exposed to zymolyase
This SuperSeries is composed of the following subset Series: GSE9930: Wild type 3 hour exposure to zymolyase GSE9931: hog1 mutant 3 hour exposure to zymolyase GSE9932: slt2 mutant 3 hour exposure to zymolyase GSE9933: rlm1 mutant 3 hour exposure to zymolyase GSE9934: msn2/4 mutant 3 hour exposure to...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-19 | E-GEOD-12684 | biostudies-arrayexpress
Transcriptomicswhole reference dataset (cell wall chip)
Data processing included: 1. Filtering of those bad quality image spots (these includes alterations on the shape or hybridization) 2. Filtering of low intensity which was done flagging with -50 those spots whose feature intensity was lower in any of both channels than the average of the local back...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-2105 | biostudies-arrayexpress
TranscriptomicsGlobal transcriptional response to transient cell wall damage
Data processing included: 1. Filtering of those bad quality image spots (these includes alterations on the shape or hybridization) 2. Filtering of low intensity which was done flagging with -50 those spots whose feature intensity was lower in any of both channels than the average of the local back...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-959 | biostudies-arrayexpress
TranscriptomicsHog1 mutant 4 hours exposure to congo red (cell wall chip)
S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same c...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-2107 | biostudies-arrayexpress
TranscriptomicsWild type 4 hours exposure to congo red (cell wall chip)
S. cerevisiae was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-trea...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-2106 | biostudies-arrayexpress
TranscriptomicsWild type yellow experiment (cell wall chip)
S. cerevisiae was grown on YEPD. For yellow experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 6h. At this time cells were collected. Experiment was carried out hybridising the cDNA from the same ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-2108 | biostudies-arrayexpress
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