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TranscriptomicsConserved generation of short products at piRNA loci
We analyzed small RNAs from three mammalian species, and found that in all these species piRNA-directed targeting is accompanied by the generation of short sequences that have a very precisely defined length and a specific spatial relationship with the guide piRNAs. small RNA-seq of testes lysate (b...
ORGANISM(S): Mus musculus 
2011-03-07 | E-GEOD-26160 | biostudies-arrayexpress
TranscriptomicsMIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]
Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcr...
ORGANISM(S): Mus musculus 
2011-11-30 | E-GEOD-32184 | biostudies-arrayexpress
TranscriptomicsMIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]
Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcr...
ORGANISM(S): Mus musculus 
2011-11-30 | E-GEOD-32180 | biostudies-arrayexpress
TranscriptomicsA role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing
Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in...
ORGANISM(S): Bombyx mori 
2012-09-04 | E-GEOD-39203 | biostudies-arrayexpress
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