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MetabolomicsFuel for the Damage Induced"""": Untargeted Metabolomics in Elite Rugby Union Match Play
The metabolic perturbations caused by competitive rugby are not well characterized. Our aim is to utilize untargeted metabolomics to develop appropriate interventions, based on the metabolic fluctuations that occur in response to this collision-based team sport. Seven members of an English Premiersh...
2021-09-06 | MTBLS2967 | MetaboLights
TranscriptomicsRNA messengers translated at the ER membrane
In order to identify what RNA messengers are being translated at the ER membrane, membrane-associated polysomes were separated from free polysomes by subcellular fractionation of WT and delta-egd1/delta-egd2 yeast strains and associated RNA was isolated and then identified by microarray analysis. Se...
ORGANISM(S): Saccharomyces cerevisiae 
2013-04-05 | E-GEOD-45795 | biostudies-arrayexpress
MetabolomicsNext-generation biomonitoring of the early-life chemical exposome in neonatal and infant development

Exposure to synthetic and natural chemicals is a major environmental risk factor in the etiology of many chronic diseases. Investigating complex co-exposures is necessary for a holistic assessment in exposome-wide association studies. In this work, a sensitive liquid chromatography-tandem mass sp...

2023-10-26 | MTBLS2442 | MetaboLights
TranscriptomicsIdentification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance
This SuperSeries is composed of the following subset Series: GSE11079: mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected GSE11080: Expression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock GSE11081: miRNA arrays: Ago2 transfected vs. ...
ORGANISM(S): Homo sapiens 
2008-04-07 | E-GEOD-11082 | biostudies-arrayexpress
TranscriptomicsPolysomes
Translational profiles of wild type yeast strains were identified by microarray analysis by isolating polysomes using sucrose gradients to recover polysome-associated RNA and comparing polysome-associated RNA vs total RNA Set of arrays that are part of repeated experiments
ORGANISM(S): Saccharomyces cerevisiae 
2013-04-05 | E-GEOD-45797 | biostudies-arrayexpress
TranscriptomicsNF-κB target gene microarray in HAECs in response to Pseudomonas aeruginosa
An NF-κB target gene array (Panomics, Inc., Redwood City, CA) was performed to profile the PAO1-dependent expression pattern of 111 NF-kB-regulated genes in primary human airway epithelial cells (HAECs). A small scale microarray was carried out using NF-κB target gene array kit (Panomics, Inc., Redw...
ORGANISM(S): Homo sapiens 
2007-09-08 | E-GEOD-8986 | biostudies-arrayexpress
TranscriptomicsExpression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock
HEK293T cell expression data from four conditions: mock transfection, Ago2 transfection, Ago2 & miR-1 transfection, and Ago2 & miR-124 transfection. RNA was isolated directly from 10cm dishes using Trizol reagent and amplified. Set of arrays that are part of repeated experiments Compound Based Treat...
ORGANISM(S): Homo sapiens 
2008-04-07 | E-GEOD-11080 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected
As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resi...
ORGANISM(S): Homo sapiens 
2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress
TranscriptomicsQuantitative genome-wide enhancer activity maps for five Drosophila species show functional enhancer conservation and turnover during cis-regulatory evolution
Phenotypic differences between closely related species are thought to arise primarily from changes in gene expression due to mutations in cis-regulatory sequences (enhancers). However, it has remained unclear, how frequently mutations alter enhancer activity or create functional enhancers de novo. H...
ORGANISM(S): Drosophila willistoni 
2014-04-28 | E-GEOD-48251 | biostudies-arrayexpress
TranscriptomicsRNA coimmunoprecipitations
Nascent polypeptides emerging from the ribosome during biogenesis can interact with many chaperones and protein homeostasis factors. The high sensitivity of RNA identification can be used to identify substrates of specific cotranslationally acting chaperones. Protein A-tagged (TAP-tag) chaperones as...
ORGANISM(S): Saccharomyces cerevisiae 
2013-04-05 | E-GEOD-45794 | biostudies-arrayexpress
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