HEK293T cells were collected and lysed by incubating it in lysis buffer containing 50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM NaF, 1X protease inhibitor cocktail, 1X phosphatase inhibitor cocktail for 45 mins at 4 degree C followed by centrifugation at 8000g for 10 mins at 4 degree C. Cell ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
