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TranscriptomicsIdentification of therapeutic targets for glioblastoma by network analysis
In this study, we confirmed that transformed dedifferentiated astrocytes and neurons acquired a stem/progenitor cell state, although they still retained gene expression memory from their parental cell-type. Transcriptional network analysis on transformed cells revealed up-regulation of genes involve...
ORGANISM(S): Mus musculus 
2015-05-11 | E-GEOD-64411 | biostudies-arrayexpress
TranscriptomicsQuantitative Mammalian Transcriptomics using Designed Primer-based Amplification
We describe a novel quantitative cDNA expression profiling strategy, involving amplification of the majority of mouse transcriptome using a defined set of 44 heptamer primers. The amplification protocol allows for efficient amplification from as low as 50pg of mRNA and did not alter the expression o...
ORGANISM(S): Mus musculus 
2013-04-29 | E-GEOD-45474 | biostudies-arrayexpress
TranscriptomicsTechnical Variations in Low-Input RNA-seq Methodologies
Transcriptomics data obtained from limiting amounts of mRNA is often noisy, providing primarily qualitative changes in transcript expressions. So far, technical variations arising out of the library preparation protocols have not been adequately characterized at reduced levels of mRNA. Here, we gene...
ORGANISM(S): Mus musculus 
2014-01-13 | E-GEOD-50856 | biostudies-arrayexpress
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