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Gene expression measurements in Thp, Th1 and Th2 cells polarised from naIve CD4+ T-cells isolated from wildtype and T-bet fl/fl x Cd4-Cre BALB/c mice or from WT and Gata-3 fl/fl x Tnfrsf4-Cre C57BL/6 mice.
ORGANISM(S): Mus musculus 
Human naïve CD4+ T cells (CD4+ CD45RA+ CD25- CD45RO- CD8- CD14- CD15- CD16- CD19- CD34- CD36- CD56- CD123- TCRγ/δ- HLA-DR- and CD235a-) were magnetically negatively isolated from peripheral blood. Cells were stimulated with anti-CD3/anti-CD28 antibodies plus IL-2, and samples were taken at 6h, 24h, ...
ORGANISM(S): Homo Sapiens (human) 
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