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TranscriptomicsGene expression during mouse OS25 ES cell differentiation
Position within chromosome territories, and localisation at transcription factories, are two facets of nuclear organisation that have been associated with active gene expression. However, there is still debate about whether this organisation is a cause or consequence of transcription. We induced loo...
ORGANISM(S): Mus musculus 
2010-06-25 | E-GEOD-15166 | biostudies-arrayexpress
TranscriptomicsExpression response of human monocyte derived macrophages to dexamethasone over a 24h time series
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Homo sapiens 
2015-12-17 | E-GEOD-61880 | biostudies-arrayexpress
TranscriptomicsExpression data from a 24h time series of dexamethasone treatment for mouse bone marrow derived macrophages
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Mus musculus 
2015-12-17 | E-GEOD-61879 | biostudies-arrayexpress
GenomicsGenome wide binding of glucocorticoid receptor in dexamethasone treated human monocyte derived macrophages
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Homo sapiens 
2015-12-17 | E-GEOD-61878 | biostudies-arrayexpress
GenomicsGenome wide binding of glucocorticoid receptor in dexamethasone treated mouse bone marrow derived macrophages
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid rec...
ORGANISM(S): Mus musculus 
2015-12-17 | E-GEOD-61877 | biostudies-arrayexpress
TranscriptomicsChIP for H3K27me3 in Murine ES Cells: wild type and Ring1B-/- cells
Native ChIP on chip for H3K27me3 in murine ES cells comparing WT and Ring1B-/- cells. Paper Abstract: How polycomb group proteins repress gene expression in vivo is not known. Whilst histone modifying activities of the polycomb repressive complexes have been studied extensively, in vitro data has su...
ORGANISM(S): Mus musculus 
2010-05-14 | E-GEOD-20213 | biostudies-arrayexpress
TranscriptomicsMurine Embryonic Stems Cells: H3K27me3 in Undifferentiated (UD) and Day 3 (D3) differentiated ES cells
ChIP on chip for H3K27me3 in murine ES cells comparing Undifferentiated and Day 3 differentiated. Paper Abstract: How polycomb group proteins repress gene expression in vivo is not known. Whilst histone modifying activities of the polycomb repressive complexes have been studied extensively, in vitro...
ORGANISM(S): Mus musculus 
2010-05-14 | E-GEOD-20201 | biostudies-arrayexpress
TranscriptomicsCatalytically inactive Ring1B maintains near wildtype levels of gene expression in mESCs
This experiment was designed to determine the extent of gene misregulation in mESCs containing catalytically dead Ring1B in comparison to mESCs lacking Ring1B. Polyadenylated mRNA was prepared from wildtype mESCs (WT), mESCs deficient for Ring1B (KO) and mESCs cells containing catalytically dead Rin...
ORGANISM(S): Mus musculus 
2015-09-20 | E-GEOD-69824 | biostudies-arrayexpress
GenomicsMouse ES cells expressing catalytically inactive Ring1B display impaired Ring1B and H3K27me3 deposition.
ChIP-seq for H3K27me3 and Ring1B was performed in WT mESCs and mESCs containing catalytically inactive Ring1B (I53A mutant). Cells expressing catalytically inactive Ring1B maintain the spatial distribution of Ring1B and H3K27me3 but at reduced levels. These findings support the notion that PRC2 recr...
ORGANISM(S): Mus musculus 
2015-09-20 | E-GEOD-69955 | biostudies-arrayexpress
TranscriptomicsSpatial genome organization: contrasting views from chromosome conformation capture and fluorescence in situ hybridization
Though important for gene regulation most studies of genome organisation use either fluorescence in situ hybridisation (FISH) or chromosome conformation capture (3C) methods. FISH directly visualises the spatial relationship of sequences, but is usually applied to a few loci at a time. The frequency...
ORGANISM(S): Mus musculus 
2015-01-02 | E-GEOD-61814 | biostudies-arrayexpress
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