Umkirch-1/Umkirch-3 hybrid plants and their parents were grown at 23SD and then shifted to 16SD for five days. 10 plants were pooled in each of three sample replicates.
Umkirch-1/Umkirch-3 hybrid plants (KB142-2) grown at 16 degreeC long day were compared with siblings carrying a microRNA targeting the Umkirch-3 allele of At5g41750. Plants were about 3 weeks old, and whole rosettes were harvested. Each sample is a pool of 8-10 individuals.
Bla-1, Hh-0 and their F1 hybrid, and Mir-0, Se-0 and their F1 hybrid were grown for 3 weeks at 16 degreC long days. Whole rosettes were harvested and pooled 8-10 per sample.
KZ10/Mrk-0 F1 hybrids and their parents were grown at 23 degreeC long days for 12 days then shifted to 16 degreeC and harvested after 2 days. Whole rosettes were harvested, and each sample is a pool of ten plants.
