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ProteomicsThe Aged Neuronal Aggregome Identified by BONCAT
Aggregating neuronal proteins. The identification of neuronal proteins contributing to aged protein aggregates was enabled by labeling the neuronal proteome with BONCAT technology, enrichment of insoluble protein aggregates, and affinity enrichment for the tagged proteins.
ORGANISM(S): Mus musculus (Mouse) 
2025-11-07 | PXD066053 | Pride
Boncat Aggregates Neurons Aggregome 
ProteomicsEnrichment and quantitation of newly secreted proteins under serum condition by combining BONCAT and pulsed SILAC
Despite the increasing interest of secretome involved in paracrine/autocrine mechanisms, the majority of the mass spectrometric cell secretome studies have been performed using serum-free medium (SFM), due to low-abundance of secreted proteins and high-abundance of contaminating fetal bovine serum (...
ORGANISM(S): Homo sapiens (Human) 
2019-03-13 | PXD007529 | Pride
Boncat Serum Fbs Secretome Pulsed silac Lc-ms/ms 
ProteomicsPseudomonas aeruginosa BONCAT biofilms
Cell-selective BONCAT analysis of proteins synthesized by a P. aeruginosa biofilm subpopulation throughout treatment with ciprofloxacin.
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) 
2017-10-24 | PXD007261 | Pride
Boncat Ciprofloxacin Pseudomonas aeruginosa Biofilms 
ProteomicsTime-resolved proteomic analysis in zebrafish larvae using bioorthogonal noncanonical amino acid tagging (BONCAT)
Protein synthesis underpins many biological processes, yet tracking time-dependent proteomic changes remains challenging. Bioorthogonal noncanonical amino acid tagging (BONCAT) offers a targeted approach for labeling and identifying newly synthesized proteins within defined time windows of interest....
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD063084 | Pride
Boncat Heat shock Larval zebrafish Noncanonical amino acid tagging Protein synthesis Proteomics Click chemistry 
ProteomicsBONCAT Model Comparison in Camk2a+ Neurons
Comparison of three different BONCAT models (MetRS, PheRS, TyrRS) driven by Camk2a promoter to drive BONCAT machinery in excitatory neurons.
ORGANISM(S): Mus musculus (Mouse) 
2025-11-07 | PXD057020 | Pride
Boncat Metrs Phers Tyrrs 
ProteomicsTracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Metaproteomics enables the description of microbial communities (MC). Microbial adaptation to changing environments is conducted by expressing newly synthesized proteins (nP) that can be difficult to distinguish from background proteins. Focusing on nP would add a new dimension to the metaproteomics...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) Saccharomyces cerevisiae (Baker's yeast) anaerobic digester metagenome 
2024-10-17 | PXD047252 | Pride
Boncat Anaerobic digestion Metaproteomics Microbiome Mass spectrometry Click chemistry 
ProteomicsComparison of Transgenic and Viral Labeling
Comparison of BONCAT labeling in Camk2aCre;PheRS* transgenic mice to mice injected with AAV-Camk2a;PheRS*.
ORGANISM(S): Mus musculus (Mouse) 
2025-11-07 | PXD057456 | Pride
Transgenic Viral Boncat 
ProteomicsPseudomonas aeruginosa BONCAT biofilms - rpoS vs. trc
Cell-selective BONCAT analysis of proteins synthesized by two P. aeruginosa biofilm subpopulations.
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) 
2017-10-24 | PXD007622 | Pride
Boncat Pseudomonas aeruginosa Biofilms 
ProteomicsCell-specific non-canonical amino acid labelling identifies changes in the hippocampal de novo proteome during memory formation
The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-type-specific labelling of newly synthesised ...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-26 | PXD015820 | Pride
Long-term memory Non-canonical amino acid labelling Azidohomoalanine Azidonorleucine Swath Active place avoidance Protein synthesis Mrna splicing 
UnknownProbing the active fraction of soil microbiomes using BONCAT-FACS.
Not available
S-EPMC6591230 | biostudies-literature
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