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1 x 106 PC12-CCK-2R cells/100 mm or 1 x 106 PC12 cells/100 mm were seeded in DMEM containing 0.5% serum for 2 days before cells were untreated or treated with 200 nM CCK-8S for 2, 4, 8, and 16 hours. Total cellular RNA was isolated. Three independent series containing RNA from four 100 mm petri dis...
ORGANISM(S): Rattus norvegicus 
The objective of this study was to investigate the differences in the gene expression profile of the granulosa cells from preovulatory follicles obtained after controlled ovarian hyperstimulation (COH) with either recombinant FSH (rFSH) or urine derived human menopausal gonadotropins (hMG).
ORGANISM(S): Homo sapiens 
Skeletal muscle has been identified as a secretory organ. We hypothesized that IL-6, a cytokine secreted from skeletal muscle during exercise, could induce production of other secreted factors in skeletal muscle. Keywords: Human skeletal muscle time course response to IL-6 infusion IL-6 was infused...
ORGANISM(S): Homo sapiens 
The aim of the study was to define the specific gene expression signature of pancreatic endoderm cells (PECs), by designing a strategy for generating putative PECs (PDX1+/NKX6-1+, and posterior foregut endoderm (PFG) cells (PDX1+/NKX6-1-). Cell line: PDX1-eGFP reporter cell line (PDXeG clone 170-3...
ORGANISM(S): Homo sapiens 
To identify factors and pathways regulated by IMP proteins and obtain leads to the mechanism behind the phenotypic changes, we compared the gene expression profiles of IMP siRNA treated cells with mock treated cells. Triplicate gene expression profiles were generated from both the IMP(1,3)A and IMP(...
ORGANISM(S): Homo sapiens 
Fresh, human CD138+ bone marrow plasma cells from multiple myeloma patients: newly diagnosed high-dose treatment candidates (N, n=29), previously high-dose treated patients experiencing first-time progressive disease (R1, n=27) or later (R2+, n=11). Gene expression compared with clinical data, inclu...
ORGANISM(S): Homo sapiens 
Isolation of IMP1 bound mRNAs. Flag-tagged IMP1 was expressed in HEK293 cells. Flag tagged IMP1 was immunoprecipitated and mRNAs isolated. As controls HEK293 cells that do not express Flag-tagged IMP1 was included.
ORGANISM(S): Homo sapiens 
Emergency granulopoiesis refers to the increased production of neutrophils in bone marrow and their release into circulation induced by severe infection. Several studies point to a critical role for granulocyte colony-stimulating factor (G-CSF) as the main mediator of emergency granulopoiesis. Howev...
ORGANISM(S): Homo sapiens 
Comparison of 9 paired human granulosa cell samples just before and 36h after hCG triggering for controlled ovarian stimulation, to explore genes regulated by hCG and involved with ovulation and final oocyte maturation.
ORGANISM(S): Homo sapiens 
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