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TranscriptomicsStudying changes in chromatin accessibility in A549 cells by ATAC-seq directly after 4-hour glucocorticoid treatment and following a 24-hour hormone washout
We performed ATAC-seq in the A549 cell line. Cells were treated with dexamethasone (100 nM), hydrocortisone (100 nM) or vehicle (ethanol) for 4 hours and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest.
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9911 | biostudies-arrayexpress
TranscriptomicsStudying changes in chromatin accessibility in A549 cells by ATAC-seq directly after 20-hour glucocorticoid treatment and following a 24-hour hormone washout
We performed ATAC-seq in the A549 cell line. Cells were treated with dexamethasone (100 nM), hydrocortisone (100 nM) or vehicle (ethanol) for 20 hours and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest.
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9910 | biostudies-arrayexpress
TranscriptomicsCircularized chromosome conformation capture (4C-seq) in A549 cells after glucocorticoid re-induction
We performed 4C-seq in the A549 cell line to investigate changes in chromatin interactions at the ZBTB16 locus. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were t...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9915 | biostudies-arrayexpress
TranscriptomicsStudying changes in chromatin accessibility in A549 cells by ATAC-seq after glucocorticoid re-induction
We performed ATAC-seq in the A549 cell line. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% e...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9912 | biostudies-arrayexpress
TranscriptomicsStudying changes in chromatin accessibility in U2OS-GR cells by ATAC-seq directly after 4-hour glucocorticoid treatment and following a 24-hour hormone washout
We performed ATAC-seq in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. Cells were treated with dexamethasone (100 nM) or vehicle (ethanol) for 4 hours and either harvested immediately or washed 2x with PBS and su...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9909 | biostudies-arrayexpress
TranscriptomicsATAC-seq in the U2OS-AR cell line comparing cells treated for 4 hours with 5 nM R1881 to vehicle (DMSO) control-treated cells
We performed ATAC-seq in the U2OS-AR cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for human AR), upon androgen (R1881) or vehicle (DMSO) treatment for 4 hours.
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9606 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of H3K27ac, H3K4me1, H3K27me3 and H3K9me3 (ChIP-seq) in U2OS-GR and U2OS-AR cells
We performed ChIP-seq targeting the H3K27ac, H3K4me1, H3K27me3 and H3K9me3 in the U2OS-GR and U2OS-AR cell lines. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2OS-GR cells were treated with dexa...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9617 | biostudies-arrayexpress
TranscriptomicsStudying enhancer activity by FAIRE-STARR-seq in the U2OS-GR and the U2OS-AR cells upon hormone treatment
To quantify functional enhancers, we performed STARR-seq (Self-Transcribing Active Regulatory Region sequencing) in the U2OS-GR and the U2OS-AR cell lines (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for rat GR or human AR, respectively). U2OS-GR cells were treated...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9614 | biostudies-arrayexpress
TranscriptomicsGlucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment
We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line and the androgen receptor (AR) in the U2OS-AR cell line. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2O...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9616 | biostudies-arrayexpress
TranscriptomicsATAC-seq in GR18 cell line comparing cells treated for 90 minutes with 1 micro molar dexamethasone with vehicle (ethanol) control-treated cells
We performed ATAC-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment for 90 minutes.
ORGANISM(S): Homo sapiens 
2019-03-11 | E-MTAB-7746 | biostudies-arrayexpress
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