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TranscriptomicsEarly and late response of Nematostella vectensis transcriptome to heavy metals
Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians, dating back to 700 million years ago, play an important role in shapi...
ORGANISM(S): Nematostella vectensis 
2015-12-22 | E-GEOD-58769 | biostudies-arrayexpress
ProteomicsDIA-PASEF–based total proteome profiling of HeLa cells expressing nuclear or cytosolic p62 condensates
Cells were lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 × g for 15 min at 4 °C. The supernatants were recovered, and proteins (20 µg each) were purified by the SP3 method an...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072518 | JPOST Repository
ProteomicsDIA-PASEF–based FLAG-IP proteomic analysis of nuclear or cytosolic p62 condensates in HeLa cells
Cells were cross-linked with 0.1% formaldehyde for 10 min at 25 °C and quenched with 100 mM glycine. After washing with HBS (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl), cells were lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM MgCl₂, 1 mM EGTA, 1% NP-40, 0.25% sodium deoxycholat...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072519 | JPOST Repository
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