Differentiated human enterocytic Caco-2/TC7 cells grown on transwell were incubated with LXR agonist GW3965 (5µM) or Vehicle DMSO (1:2000 ; vol:vol) for 48 hours. Total RNA were extracted using mirVana miRNA Isolation Kit (life technologies) then processed with Flash Tag Biotin HSR kit (Affymetrix) ...
To identify genes regulated by FXR in GLUTag cell line, we compared gene expression profiles in GLUTag cells treated for 24h by 0.1% DMSO and by 5M-5M GW4064
