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TranscriptomicsRNA-seq analysis of ES cell populations with different functional potentials in serum/LIF and 2i/LIF, as well as analysis of the transcriptional effect of LIF in ground state (2i) culture conditions.
We report that ES cells cultured in ground state (2i and 2i/LIF) culture conditions are heterogeneous and show heterogeneus expression of extraembryonic markers. Using a highly sensitive reporter for the endoderm marker Hex we can sort Hex high and low populations from either serum/LIF or 2i/LIF and...
ORGANISM(S): Mus musculus 
2013-06-11 | E-GEOD-45182 | biostudies-arrayexpress
TranscriptomicsXenopus laevis embryos and animal caps: Control vs PouV morpholino
Transcriptional profiling of Xenopus laevis embryos and ectoderm (animal caps) comparing embryos injected with control morpholino with embryos injected with the morpholino mixture PVD2, which knocks down all three Xenopus PouV proteins. Whole embryos (WE) or animal caps (AC) were collected at late b...
ORGANISM(S): Xenopus laevis 
2013-11-12 | E-GEOD-47578 | biostudies-arrayexpress
ProteomicsTime-resolved analysis reveals rapid dynamics and broad scope of the CBP/p300 acetylome
The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators; however, their acetylation targets, site-specific acetylation kinetics, and function in proteome regulation are incompletely understood. We combined quantitative proteomics with novel CBP/p300-specific catalytic i...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
MSV000095981 | MassIVE
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