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ProteomicsVariations of secretome profiles according to conditioned medium preparation: the example of human mesenchymal stem cell derived adipocytes
One challenging point in analysing cellular secretome collected as conditioned medium is cross contamination by cell culture media components, especially bovine serum proteins. A common approach for serum removal is to wash the cells, an alternative is to grow cells using serum-free conditions. Give...
ORGANISM(S): Homo sapiens (Human) 
2015-07-03 | PXD002217 | Pride
Adipocyte/conditioned medium/mesenchymal stem cell /secretome 
TranscriptomicsTranscriptomic analysis of osteoblastic cells derived from human mesenchymal stem cells, incubated with secreted factors release by adipocytes against non-incubated controls
Our preliminary findings lead us to propose bone marrow adipocyte secretions as new actors of bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocy...
ORGANISM(S): Homo sapiens 
2021-01-05 | E-MTAB-8849 | biostudies-arrayexpress
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